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D5042

123 bp DNA Ladder

Name: 123 bp DNA Ladder
Brand: SIGMA

for DNA electrophoresis

Synonym(s):

DNA Ladder for DNA electrophoresis, DNA marker

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About This Item

NACRES:

NA.25

UNSPSC Code:

41105335

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Properties

Quality Level

100, 200

form

liquid

usage

100 uses (0.1 mg size), 250 uses (250 μg size)

suitability

suitable for electrophoresis (DNA)

storage temp.

−20°C

Description

Application

123 bp DNA Ladder has been used as a size marker in random amplified polymorphic DNA-polymerase chain reaction (PCR) analysis. It has also been used as a molecular marker in gel electrophoresis.

Other Notes

Contains 35 fragments, 123 - 4182 bp

For optimal resolution, the recommended agarose gel concentration is 1.5%.

Sigma′s 123 bp DNA Ladder is provided in a solution of 10 mM Tris-HCl (pH 7.5), 50 mM NaCl, and 0.1 mM EDTA.

Storage Class

11 - Combustible Solids

wgk

WGK 3

ppe

Eyeshields, Gloves, type N95 (US)

flash_point_f

No data available

flash_point_c

No data available

Regulatory Information

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Articles

Selecting DNA, RNA, and PCR Fragment Markers and Ladders for Gel Electrophoresis

Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.

Gel Electrophoresis Markers

Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.

Water for Nucleic Acid Gel Electrophoresis

Learn how water impurities can affect DNA and RNA electrophoresis and blotting. High purity water purified using ultrafiltration is suitable for nucleic acid electrophoresis, Northern and Southern blotting.

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Global Trade Item Number

SKU	GTIN
D5042-100UG	04055977220308
D5042-250UG	04061835572717
D5042-.1MG	04061838255181