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335940

Sigma-Aldrich

Xylene Cyanol FF

Dye content ≥75 %

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Synonym(s):
Acid blue 147, Cyanol FF, XC, Xylene Cyanole FF
Empirical Formula (Hill Notation):
C25H27N2NaO6S2
CAS Number:
2650-17-1
Molecular Weight:
538.61
Colour Index Number:
42135
EC Number:
220-167-5
MDL number:
MFCD00040657
UNSPSC Code:
12171500
PubChem Substance ID:
24860353
NACRES:
NA.47

Assay

≥96.0% (HPLC)

Quality Level

200

form

powder or crystals

composition

Dye content, ≥75%

mp

295 °C (dec.) (lit.)

solubility

water: 1 mg/mL, blue

λmax

615 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].CCNc1ccc(cc1C)C(=C2/C=C\C(=N/CC)C(C)=C2)\c3ccc(cc3S(O)(=O)=O)S([O-])(=O)=O

InChI

1S/C25H28N2O6S2.Na/c1-5-26-22-11-7-18(13-16(22)3)25(19-8-12-23(27-6-2)17(4)14-19)21-10-9-20(34(28,29)30)15-24(21)35(31,32)33;/h7-15,26H,5-6H2,1-4H3,(H,28,29,30)(H,31,32,33);/q;+1/p-1/b25-19-,27-23+;

InChI key

VVLFAAMTGMGYBS-AHRQKMASSA-M

Related Categories

General description

Xylene cyanol is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis. It has a slightly negative charge and will migrate in the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The tracking dye typically migrates with the DNA molecules around 5kb. Xylene cyanol may also be used in loading buffer that facilitates the observation of the sample during gel loading and electrophoresis.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H319

Hazard Classifications

Eye Irrit. 2

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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