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B5525

Sigma-Aldrich

Bromophenol Blue sodium salt

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Synonym(s):
3′,3′′,5′,5′′-Tetrabromophenolsulfophthalein sodium salt
Empirical Formula (Hill Notation):
C19H9Br4NaO5S
CAS Number:
34725-61-6
Molecular Weight:
691.94
Beilstein:
6995701
EC Number:
252-170-2
MDL number:
MFCD00013793
UNSPSC Code:
12352200
PubChem Substance ID:
57653941
NACRES:
NA.52

form

powder

Quality Level

200

technique(s)

electrophoresis: suitable

solubility

water: soluble

suitability

suitable for nucleic acid electrophoresis

storage temp.

room temp

SMILES string

[Na+].Oc1c(Br)cc(cc1Br)C(=C2/C=C(Br)C(=O)C(Br)=C2)\c3ccccc3S([O-])(=O)=O

InChI

1S/C19H10Br4O5S.Na/c20-12-5-9(6-13(21)18(12)24)17(10-7-14(22)19(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28;/h1-8,24H,(H,26,27,28);/q;+1/p-1

InChI key

TWKDJMRHSLWRFI-UHFFFAOYSA-M

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General description

Bromophenol Blue is a pH indicator, and a dye appearing as a strong blue color. It is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis. Bromophenol blue has a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.

Application

Bromophenol blue sodium salt has been used in the preparation of protein samples for western blotting analysis. It has also been used in Drosphila fly food to aid visualization of gut for dissection.
A tracking dye for nucleic acid or protein electrophoresis in agarose or polyacrylamide gels.

Reconstitution

A 6x Gel Loading Buffer can be made using standard recipes, including 0.015% bromophenol blue.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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