SAB4500366
Anti-Collagen II antibody produced in rabbit
affinity isolated antibody
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All Photos(3)
Synonym(s):
CO2A1, chondrocalcin, collagen, collagen α-1 (II), type II
UNSPSC Code:
12352203
NACRES:
NA.43
Recommended Products
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 141 kDa
species reactivity
human, mouse, rat
concentration
~1 mg/mL
technique(s)
ELISA: 1:20000
immunofluorescence: 1:100-1:500
immunohistochemistry: 1:50-1:100
western blot: 1:500-1:1000
NCBI accession no.
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... COL2A1(1280)
General description
Anti-Collagen II Antibody detects endogenous levels of total Collagen II protein.
Collagen type II α 1 chain (COL2A1) gene is mapped to human chromosome 12q13.11–q13.12. COL2A1 belongs to collagen superfamily. The encoded protein is characterized with three identical α chains forming homotrimers. Collagen II is the major component of cartilage.
Immunogen
The antiserum was produced against synthesized peptide derived from human Collagen II.
Immunogen Range: 101-150
Immunogen Range: 101-150
Application
Anti-Collagen II, N-Terminal antibody produced in rabbit has been used for:
- Western blotting
- Immunofluorescence
- Immunohistological analyses
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (2 papers)
Immunohistochemistry (2 papers)
Biochem/physiol Actions
Mutation of collagen type II α 1 chain (COL2A1), leads to a variety of clinical manifestations such as skeletal dysplasia, short stature, and sensorial defects, collectively known as type II collagenopathies. Function of COL2A1 in articular cartilage is to form highly organized fibers, which are essential for the mechanical properties of the tissue.
Features and Benefits
Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.
Physical form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
常规特殊物品
Certificates of Analysis (COA)
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Shiping Shi et al.
Experimental and therapeutic medicine, 24(2), 526-526 (2022-07-16)
Osteoarthritis (OA) is a chronic condition caused by cartilage degradation, and there are currently no effective methods for preventing the progression of this disease; gene therapy is a relatively novel method for treating arthritis. Decreased collagen type II (Col2) expression
E V Isaeva et al.
Sovremennye tekhnologii v meditsine, 15(2), 5-16 (2023-06-30)
The aim of the study was to compare type I collagen-based and methacryloyl gelatin-based (GelMA) hydrogels by their ability to form hyaline cartilage in animals after subcutaneous implantation of scaffolds. Chondrocytes were isolated from the costal cartilage of newborn rats
Elena V Isaeva et al.
International journal of molecular sciences, 23(5) (2022-03-11)
The aim of this study was to verify the applicability of high-concentration collagen-based bioink with MSC (ADSC) and decellularized ECM granules for the formation of cartilage tissue de novo after subcutaneous implantation of the scaffolds in rats. The printability of
Yu Yao et al.
International journal of molecular medicine, 41(1), 173-183 (2017-11-09)
Surgery-obtained synovium specimens (SSSs) can provide a source of synovial mesenchymal stem cells (SMSCs) for experimental studies. However, these specimens contain diverse tissues, including the intima and subintima; therefore, these SMSCs are not entirely derived from the intima and their cell source
E Takahashi et al.
Human genetics, 86(1), 14-16 (1990-11-01)
A new mapping system, based on nonisotopic in situ hybridization combined with fluorescent staining of replicated prometaphase R-bands, is described. Replication of the bands is achieved by treatment of thymidine-synchronized cells with bromodeoxyuridine. The human COL2A1 gene was mapped to
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