一般描述
Solid Phase Reversible Immobilization (SPRI) beads are magnetic beads that can be utilized for DNA purification and size selection. The 5R-PLEX beads are SPRI beads designed to easily separate the desired PCR amplicon from nucleotides, adapters, primers, and primer dimers by efficient purification and size selection. The magnetic beads are provided in a ready to use suspension that enables fast and efficient application even in high throughput applications with automated liquid handling. The beads are formulated with NaCl and high purity PEG as the crowding agent.
应用
- NGS library preparation, including 16S and 18S
- Approved to use in ultrasensitive 5R-Plex (SMURF) library preparation(2,3,4) for degraded samples
- PCR reaction clean up
- Amplicon size selection
- DNA purification and manipulation (replacing elution buffer, concentrating the sample)
特点和优势
- Ready to use
- Compatible with automated liquid handling
- Easy size selection by changing the volumetric ratio of beads to samples
WGK
WGK 1
Lishay Parhi et al.
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Fusobacterium nucleatum is an oral anaerobe recently found to be prevalent in human colorectal cancer (CRC) where it is associated with poor treatment outcome. In mice, hematogenous F. nucleatum can colonize CRC tissue using its lectin Fap2, which attaches to
Solid-phase reversible immobilization for the isolation of PCR products.
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Stepwise remodeling and subcompartment formation in individual vesicles by three ESCRT-III proteins.
Yunuen Avalos-Padilla et al.
iScience, 26(1), 105765-105765 (2023-01-03)
The endosomal sorting complex required for transport (ESCRT) is a multi-protein machinery involved in several membrane remodeling processes. Different approaches have been used to resolve how ESCRT proteins scission membranes. However, the underlying mechanisms generating membrane deformations are still a
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