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Safety Information

P7640

Sigma-Aldrich

Phosphatase, Alkaline from bovine intestinal mucosa

lyophilized powder, ≥10 DEA units/mg solid

Synonym(s):

Alkaline phosphatase, Orthophosphoric-monoester phosphohydrolase (alkaline optimum)

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500 MG
CN¥1,494.52
1 G
CN¥2,597.52
5 G
CN¥8,191.54
10 G
CN¥16,304.83

CN¥1,494.52

Available to ship onApril 27, 2025Details

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500 MG
CN¥1,494.52
1 G
CN¥2,597.52
5 G
CN¥8,191.54
10 G
CN¥16,304.83

About This Item

CAS Number:
9001-78-9
Enzyme Commission number:
3.1.3.1 (BRENDA, IUBMB)
EC Number:
232-631-4
MDL number:
MFCD00131849
UNSPSC Code:
12352204
eCl@ss:
42010105
NACRES:
NA.54

CN¥1,494.52

Available to ship onApril 27, 2025Details

Request a Bulk Order

biological source

bovine intestinal mucosa

Quality Level

200

form

lyophilized powder

specific activity

≥10 DEA units/mg solid

mol wt

dimer ~160 kDa

shipped in

wet ice

storage temp.

−20°C

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This Item
P0114A2356P5521
Phosphatase, Alkaline from bovine intestinal mucosa lyophilized powder, ≥10 DEA units/mg solid

P7640

Phosphatase, Alkaline from bovine intestinal mucosa

Phosphatase, Alkaline from bovine intestinal mucosa BioUltra, ≥5,700 DEA units/mg protein

P0114

Phosphatase, Alkaline from bovine intestinal mucosa

Phosphatase, Alkaline from bovine intestinal mucosa ≥5,500 DEA units/mg protein

A2356

Phosphatase, Alkaline from bovine intestinal mucosa

Phosphatase, Alkaline from bovine intestinal mucosa ≥2,000 DEA units/mg protein

P5521

Phosphatase, Alkaline from bovine intestinal mucosa

specific activity

≥10 DEA units/mg solid

specific activity

≥5,700 DEA units/mg protein

specific activity

≥5,500 DEA units/mg protein

specific activity

≥2,000 DEA units/mg protein

biological source

bovine intestinal mucosa

biological source

-

biological source

-

biological source

-

form

lyophilized powder

form

(40% glycerol containing 6 mM Tris, 6 mM MgCl2 and 0.12 mM ZnCl2, pH approximately 7.6)

form

(Solution in 40% glycerol containing 6 mM Tris, 6 mM MgCl2 and 0.12 mM ZnCl2, pH approximately 7.6)

form

aqueous solution

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

mol wt

dimer ~160 kDa

mol wt

dimer ~160 kDa

mol wt

dimer ~160 kDa

mol wt

dimer ~160 kDa

shipped in

wet ice

shipped in

wet ice

shipped in

-

shipped in

-

Application

Alkaline phosphatase can be used to dephosphorylate casein and other proteins. Alkaline phosphatase may be also be used to dephosphorylate the 5′-termini of DNA or RNA to prevent self-ligation. DNA or RNA can also be tagged with radiolabeled phosphate (via T4 polynucleotide kinase) after dephosphorylation with alkaline phosphatase..
Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It is routinely used to dephosphorylate proteins and nucleic acids. It may be used for protein labeling when high sensitivity is required. Alkaline phosphatase may be also be used to dephosphorylate the 5′-termini of DNA or RNA to prevent self-ligation. DNA or RNA can also be tagged with radiolabeled phosphate (via T4 polynucleotide kinase) after dephosphorylation with alkaline phosphatase.. Product P7640 has been used to study the monoclonal alkaline phosphatase-anti-alkaline phosphatase (APAAP) complex[1].
High specific activity grade recommended for antibody and protein conjugation.

Biochem/physiol Actions

Alkaline phosphatase, from bovine intestinal mucosa, is most stable in the pH range 7.5-9.5. The enzyme has a broad specificity for phosphate esters of alcohols, amines, pyrophosphate, and phenols and it requires zinc, and magnesium or calcium divalent ions for activity.
The enzyme has a broad specificity for phosphate esters of alcohols, amines, pyrophosphate, and phenols. It is routinely used to dephosphorylate proteins and nucleic acids.

Physical properties

Bovine intestinal alkaline phosphatase is a dimeric, membrane-derived glycoprotein. At least three isoforms exist, which typically possess two N-linked and one or more O-linked glycans per monomer.
Bovine intestinal alkaline phosphatase is a dimeric, membrane-derived glycoprotein. At least three isoforms exist, which typically possess two N-linked and one or more O-linked glycans per monomer.2 The enzyme requires zinc, and magnesium or calcium divalent ions for activity.

Unit Definition

One DEA unit will hydrolyze 1 μmole of 4-nitrophenyl phosphate per minute at pH 9.8 at 37 °C. (One glycine unit is equivalent to ~3 DEA units)

Analysis Note

Package sizes are based on DEA units

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

动植物源性产品
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    Certificates of Analysis (COA)

    Lot/Batch Number
    0000428915
    0000428913
    0000428913
    0000428915
    0000418325

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    Mamoni Dash et al.
    Carbohydrate polymers, 182, 254-264 (2017-12-28)
    Polyelectrolyte complexes (PEC) of chitosan and ulvan were fabricated to study alkaline phosphatase (ALP) mediated formation of apatitic minerals. Scaffolds of the PEC were subjected to ALP and successful mineral formation was studied using SEM, Raman and XRD techniques. Investigation
    A Hohmann et al.
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 36(2), 137-143 (1988-02-01)
    A mouse monoclonal antibody, FMC55 (an IgG1), to alkaline phosphatase was prepared and evaluated in immunostaining. Clones producing antibody to alkaline phosphatase were selected using a micro-ELISA which identified antibodies forming active soluble complexes (APAAP) with the enzyme. Conditions that
    Simultaneous flow-injection determination of fluoride, monofluorophosphate and orthophosphate ions using alkaline phosphatase immobilized on a cellulose nitrate membrane and an open-circulation approach.
    Tzanavaras PD and Themelis DG
    Analytica Chimica Acta, 467 (1-2), 83-89 (2002)
    Stefanie Riedel et al.
    Journal of functional biomaterials, 12(4) (2021-10-27)
    Biological hydrogels are highly promising materials for bone tissue engineering (BTE) due to their high biocompatibility and biomimetic characteristics. However, for advanced and customized BTE, precise tools for material stabilization and tuning material properties are desired while optimal mineralisation must
    Some properties of alkaline phosphatase of cow's milk and calf intestinal mucosa.
    R K MORTON
    The Biochemical journal, 60(4), 573-582 (1955-08-01)
    View All Related Papers

    Protocols

    Diethanolamine Assay for the Enzymatic Assay of Alkaline Phosphatase (EC 3.1.3.1)

    Enzymatic Assay of Alkaline Phosphatase, Diethanolamine Assay (EC 3. 1. 3. 1)

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