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Key Documents

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Safety Information

P0114

Sigma-Aldrich

Phosphatase, Alkaline from bovine intestinal mucosa

BioUltra, ≥5,700 DEA units/mg protein

Synonym(s):

Alkaline phosphatase, Orthophosphoric-monoester phosphohydrolase (alkaline optimum)

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About This Item

CAS Number:
9001-78-9
Enzyme Commission number:
3.1.3.1 (BRENDA, IUBMB)
MDL number:
MFCD00131849
UNSPSC Code:
12352204
eCl@ss:
42010105
NACRES:
NA.54

CN¥8,017.76

Please contact Customer Service for Availability
Request a Bulk Order

product line

BioUltra

form

(40% glycerol containing 6 mM Tris, 6 mM MgCl2 and 0.12 mM ZnCl2, pH approximately 7.6)

specific activity

≥5,700 DEA units/mg protein

mol wt

dimer ~160 kDa

concentration

≥10 mg/mL

shipped in

wet ice

storage temp.

2-8°C

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Headspace vial, screw top, rounded bottom (vial only)

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SU860098

Headspace vial, screw top, rounded bottom (vial only)

Headspace vial, screw top, rounded bottom (vial only) volume 10 mL, clear glass vial, thread for 18, O.D. × H 22.5 mm × 46 mm, pkg of 100 ea

SU860099

Headspace vial, screw top, rounded bottom (vial only)

Headspace vial, beveled top, rounded bottom volume 20 mL, clear glass vial, O.D. × H × I.D. 23 mm × 75.5 mm × 12.5 mm, for use with PerkinElmer, pkg of 100 ea

SU860049

Headspace vial, beveled top, rounded bottom

material

clear glass vial, round bottom

material

amber glass vial, round bottom

material

clear glass vial, round bottom

material

clear glass vial, round bottom

fitting

thread for 18

fitting

thread for 18

fitting

thread for 18

fitting

thread for 20 mm

volume

20 mL

volume

20 mL

volume

10 mL

volume

20 mL

compatibility

for use with CTC PAL (Varian, Gerstel, Atas, Shimadzu)

compatibility

for use with CTC PAL (Varian, Gerstel, Atas, Shimadzu)

compatibility

for use with CTC HTC PAL, for use with CTC PAL (Varian, Gerstel, Atas, Shimadzu)

compatibility

for use with PerkinElmer

packaging

pkg of 100 ea

packaging

pkg of 100 ea

packaging

pkg of 100 ea

packaging

pkg of 100 ea

General description

Alkaline phosphatase from bovine intestinal mucosa is both a phosphomonoesterase and a pyrophosphatase. [1]

Application

Alkaline phosphatase can be used to dephosphorylate casein and other proteins. Alkaline phosphatase may be also be used to dephosphorylate the 5′-termini of DNA or RNA to prevent self-ligation. DNA or RNA can also be tagged with radiolabeled phosphate (via T4 polynucleotide kinase) after dephosphorylation with alkaline phosphatase..
High specific activity grade recommended for antibody and protein conjugation.
Phosphatase alkaline from bovine intestinal mucosa has been used in a study to measure the hydrolysis kinetics of three polyfluorinated alkyl phosphate monoesters, differing in fluorinated chain length. [2] It has also been used in a study to show that the developmentally controlled expression of the isosomes C-1 and C-2 indicate functional changes in intestinal alkaline phosphatase with tissue maturation. [3]

Biochem/physiol Actions

Alkaline phosphatase was shown to have anti-inflammatory effects and can reduce the severity of septic shock by detoxifying lipid A. [4][5]
The enzyme has a broad specificity for phosphate esters of alcohols, amines, pyrophosphate, and phenols. It is routinely used to dephosphorylate proteins and nucleic acids.

Physical properties

Bovine intestinal alkaline phosphatase is a dimeric, membrane-derived glycoprotein. At least three isoforms exist, which typically possess two N-linked and one or more O-linked glycans per monomer.
Bovine intestinal alkaline phosphatase is a dimeric, membrane-derived glycoprotein. At least three isoforms exist, which typically possess two N-linked and one or more O-linked glycans per monomer.2 The enzyme requires zinc, and magnesium or calcium divalent ions for activity.

Unit Definition

One DEA unit will hydrolyze 1 μmole of 4-nitrophenyl phosphate per minute at pH 9.8 at 37 °C. (One glycine unit is equivalent to ~3 DEA units)

Physical form

Solution in 40% glycerol containing 6 mM Tris, 6 mM MgCl2 and 0.12 mM ZnCl2, pH approximately 7.6

Analysis Note

Package sizes are based on DEA units

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

动植物源性产品

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    0000440965
    0000440966
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    0000440965
    0000429420

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    Suzanne Kats et al.
    Recent patents on inflammation & allergy drug discovery, 3(3), 214-220 (2009-06-19)
    Laboratory and clinical data have implicated endotoxin as an important factor in the inflammatory response to cardiopulmonary bypass. Alkaline phosphatase prevents endotoxin-induced systemic inflammation in animals and humans. We assessed the effects of the administration of bovine intestinal alkaline phosphatase
    Senem Aykul et al.
    International journal of molecular sciences, 22(16) (2021-08-28)
    Adipose tissues (AT) expand in response to energy surplus through adipocyte hypertrophy and hyperplasia. The latter, also known as adipogenesis, is a process by which multipotent precursors differentiate to form mature adipocytes. This process is directed by developmental cues that
    M Besman et al.
    The Journal of biological chemistry, 260(20), 11190-11193 (1985-09-15)
    Alkaline phosphatases from calf and bovine small intestines have been isolated in homogeneous form from both mucosa and luminal contents. The detergent-solubilized calf enzyme resolves into two peaks of activity, C-1 and C-2, on chromatofocusing. Only one of these activity
    Dagmara Wojcik-Grzybek et al.
    International journal of molecular sciences, 23(6) (2022-03-26)
    Inflammatory bowel diseases (IBD) are commonly considered as Crohn's disease and ulcerative colitis, but the possibility that the alterations in gut microbiota and oxidative stress may affect the course of experimental colitis in obese physically exercising mice treated with the
    Fuhong Su et al.
    Critical care medicine, 34(8), 2182-2187 (2006-06-16)
    Alkaline phosphatase may decrease the harmful effects of lipopolysaccharide by detoxifying lipid A. The aim of this study was to investigate whether administration of alkaline phosphatase is beneficial in a clinically relevant septic shock model. Interventional laboratory study. University hospital
    View All Related Papers

    Protocols

    Diethanolamine Assay for the Enzymatic Assay of Alkaline Phosphatase (EC 3.1.3.1)

    Enzymatic Assay of Alkaline Phosphatase, Diethanolamine Assay (EC 3. 1. 3. 1)

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