M3168
4-Methylumbelliferyl phosphate
alkaline phosphatase substrate, liquid
Synonym(s):
4-methylumbelliferone phosphate Liquid Substrate System
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About This Item
CAS Number:
UNSPSC Code:
12352204
PubChem Substance ID:
NACRES:
NA.32
EC Number:
222-137-7
MDL number:
Product Name
4-Methylumbelliferyl phosphate (4-MUP) Liquid Substrate System, liquid
SMILES string
CC1=CC(=O)Oc2cc(OP(O)(O)=O)ccc12
InChI key
BCHIXGBGRHLSBE-UHFFFAOYSA-N
InChI
1S/C10H9O6P/c1-6-4-10(11)15-9-5-7(2-3-8(6)9)16-17(12,13)14/h2-5H,1H3,(H2,12,13,14)
form
liquid
storage temp.
2-8°C
Quality Level
Related Categories
Analysis Note
4-MUP fluorescence can be measured with excitation at 360 nm and emission at 440 nm. The fluorescent end product may also be observed using a UV light source.
Application
4-Methylumbelliferyl phosphate (4-MUP) Liquid Substrate System has been used as a fluorescent substrate for alkaline phosphate in enzyme linked immunosorbent assay (ELISA). It has also been used in secreted embryonic alkaline phosphatase (SEAP) activity assay.
General description
4-Methylumbelliferyl phosphate (4-MUP) is a substrate for alkaline phosphatase which forms the soluble fluorescent substance methylumbelliferone. 4-MUP Liquid Substrate System combines 4-MUP and buffer in a ready-to-use reagent.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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As part of a program to develop better PTP1B fluorogenic substrates that more closely mimic the functionality found in the natural substrate, we have prepared and evaluated nine novel analogs of 4-methylumbelliferone phosphate (MUP) with a variety of additional groups
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