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M3168

Sigma-Aldrich

4-Methylumbelliferyl phosphate

alkaline phosphatase substrate, liquid

Synonym(s):

4-methylumbelliferone phosphate Liquid Substrate System

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100 ML
CN¥2,621.71

CN¥2,621.71

Estimated to ship onApril 07, 2025Details

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100 ML
CN¥2,621.71

About This Item

CAS Number:
3368-04-5
EC Number:
222-137-7
MDL number:
MFCD00016969
UNSPSC Code:
12352204
PubChem Substance ID:
24896808
NACRES:
NA.32

CN¥2,621.71

Estimated to ship onApril 07, 2025Details

New, lower price on this item!

Product Name

4-Methylumbelliferyl phosphate (4-MUP) Liquid Substrate System, liquid

form

liquid

storage temp.

2-8°C

SMILES string

CC1=CC(=O)Oc2cc(OP(O)(O)=O)ccc12

InChI

1S/C10H9O6P/c1-6-4-10(11)15-9-5-7(2-3-8(6)9)16-17(12,13)14/h2-5H,1H3,(H2,12,13,14)

InChI key

BCHIXGBGRHLSBE-UHFFFAOYSA-N

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This Item
P7998N7653AB0100
4-Methylumbelliferyl phosphate (4-MUP) Liquid Substrate System liquid

M3168

4-Methylumbelliferyl phosphate (4-MUP) Liquid Substrate System

Alkaline Phosphatase Yellow (pNPP) Liquid Substrate System for ELISA ready to use solution

P7998

Alkaline Phosphatase Yellow (pNPP) Liquid Substrate System for ELISA

p-Nitrophenyl Phosphate Liquid Substrate System liquid

N7653

p-Nitrophenyl Phosphate Liquid Substrate System

Alkaline Phosphatase Blue Microwell Substrate sufficient for 400 mL working substrate

AB0100

Alkaline Phosphatase Blue Microwell Substrate

form

liquid

form

liquid

form

liquid

form

solution

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

General description

4-Methylumbelliferyl phosphate (4-MUP) is a substrate for alkaline phosphatase which forms the soluble fluorescent substance methylumbelliferone. 4-MUP Liquid Substrate System combines 4-MUP and buffer in a ready-to-use reagent.

Application

4-Methylumbelliferyl phosphate (4-MUP) Liquid Substrate System has been used as a fluorescent substrate for alkaline phosphate in enzyme linked immunosorbent assay (ELISA).[1][2][3] It has also been used in secreted embryonic alkaline phosphatase (SEAP) activity assay.[4]

Analysis Note

4-MUP fluorescence can be measured with excitation at 360 nm and emission at 440 nm. The fluorescent end product may also be observed using a UV light source.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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    Lot/Batch Number
    0000330515
    0000330515
    SLCM6733
    SLCF6247
    SLCB8519

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