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N2770

Sigma-Aldrich

p-Nitrophenyl phosphate

SIGMAFAST™, chromogenic, tablet

Synonym(s):

pNPP tablets

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About This Item

EC Number:
224-246-5
MDL number:
MFCD00284586
UNSPSC Code:
12352204
NACRES:
NA.83

Product Name

SIGMAFAST™ p-Nitrophenyl phosphate Tablets, tablet, To prepare 20 mL

Quality Level

200

form

tablet

solubility

water: 0.25 tablet/mL, clear, colorless

storage temp.

−20°C

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Application

Developed for use as a soluble substrate for the detection of alkaline phosphatase activity in Enzyme Immunoassays (EIA). pNPP is the substrate of choice in high sensitivity alkaline phosphatase-based EIA assays.One pNPP tablet and one Tris buffer tablet are dissolved in deionized water and provide a ready-to-use substrate. Each SIGMAFAST pNPP tablet set yields a solution containing 1.0 mg/ml pNPP, 0.2 M Tris buffer. EIA applications using pNPP may be read in timed assays or stopped with 3N NaOH for delayed readings. SIGMAFAST™ require only the addition of distilled or deionized water to prepare an active substrate solution. Tablets are individually packaged in foil packets.

Reconstitution

Each tablet set dissolved in 20 mL deionized water yields a ready-to-use buffered solution containing pNPP.

Legal Information

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000333606
0000333608
0000296290
0000296291
0000296291

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To fulfill the properties of membrane for guided bone tissue regeneration, chitosan (CS) and calcium phosphates were blended to produce porous hybrid membranes by lyophilization. We synthesized three different calcium phosphates: calcium deficient hydroxyapatite (CDHA), biphasic calcium phosphate (BCP) and
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