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Safety Information

79390

Sigma-Aldrich

Phosphatase, Alkaline from calf intestinal mucosa

suitable for enzyme immunoassay, solution (clear, colorless), ~2500 U/mg protein (~10 mg/ml)

Synonym(s):

Alkaline Phosphatase from calf intestinal mucosa

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1 MG
CN¥2,899.33
5 MG
CN¥10,239.43

CN¥2,899.33

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1 MG
CN¥2,899.33
5 MG
CN¥10,239.43

About This Item

CAS Number:
9001-78-9
Enzyme Commission number:
3.1.3.1 (BRENDA, IUBMB)
EC Number:
232-631-4
MDL number:
MFCD00131849
UNSPSC Code:
12352204
eCl@ss:
42010105
NACRES:
NA.54

CN¥2,899.33

Please contact Customer Service for Availability
New, lower price on this item!
Request a Bulk Order

biological source

bovine (calf) intestinal mucosa

form

solution (clear, colorless)

specific activity

~2500 U/mg protein (~10 mg/ml)

mol wt

Mr ~140000

technique(s)

enzyme immunoassay: suitable

impurities

0.1 mM ZnCl2
1 mM MgCl2
3 M NaCl
30 mM triethanolamine

pH

~7.6

storage temp.

2-8°C

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This Item
P6774A2356P5521
Phosphatase, Alkaline from calf intestinal mucosa suitable for enzyme immunoassay, solution (clear, colorless), ~2500 U/mg protein (~10 mg/ml)

79390

Phosphatase, Alkaline from calf intestinal mucosa

Phosphatase, Alkaline from bovine intestinal mucosa buffered aqueous solution, ≥2,000 DEA units/mg protein

P6774

Phosphatase, Alkaline from bovine intestinal mucosa

Phosphatase, Alkaline from bovine intestinal mucosa ≥5,500 DEA units/mg protein

A2356

Phosphatase, Alkaline from bovine intestinal mucosa

Phosphatase, Alkaline from bovine intestinal mucosa ≥2,000 DEA units/mg protein

P5521

Phosphatase, Alkaline from bovine intestinal mucosa

technique(s)

enzyme immunoassay: suitable

technique(s)

-

technique(s)

-

technique(s)

-

biological source

bovine (calf) intestinal mucosa

biological source

bovine intestinal mucosa

biological source

-

biological source

-

specific activity

~2500 U/mg protein (~10 mg/ml)

specific activity

≥2,000 DEA units/mg protein

specific activity

≥5,500 DEA units/mg protein

specific activity

≥2,000 DEA units/mg protein

form

solution (clear, colorless)

form

buffered aqueous solution

form

(Solution in 40% glycerol containing 6 mM Tris, 6 mM MgCl2 and 0.12 mM ZnCl2, pH approximately 7.6)

form

aqueous solution

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

pH

~7.6

pH

-

pH

-

pH

-

Application

Alkaline phosphatase is used in the laboratory to remove phosphate monoesters which prevents self-ligation. It is also used in the dairy industry to confirm pasturization of milk. Alkaline phosphatse is commonly conjugated to antibodies and used in immunoblotting, ELISA′s, and other antibody based detection assays.

Biochem/physiol Actions

Alkaline phosphatase is a hydrolytic enzyme which dephosphorylates a variety of molecules under basic conditions.

Unit Definition

1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol 4-nitrophenyl phosphate per minute at pH 9.8 and 37 °C

Reconstitution

The solution can be used without dialysis (for enzyme immuno-assay).

Other Notes

Sales restrictions may apply
Used in highly sensitive (pg/m) enzyme immunoassays with LCEC detection of phenol[1]; Comparison of three methods for conjugating alkaline phosphatase to human placental lactogen[2]; Preparation of enzyme-antibody conjugates[3][4]; Liposome immunoassay with antigen-coupled liposomes containing alkaline phosphatase[5]

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

动植物源性产品

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    Certificates of Analysis (COA)

    Lot/Batch Number
    BCCM6739
    BCCM0561
    BCCK7702
    BCCG6707
    BCBZ8955

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    M.I. Halliday et al.
    Biochemical Society Transactions, 14, 473-473 (1986)
    D G Williams
    Journal of immunological methods, 72(1), 261-268 (1984-08-03)
    One of the requirements for enzyme immunoassay is the formation of a labelled component of the assay system which can be either the ligand or the binder. Three methods for conjugating alkaline phosphatase to human placental lactogen were investigated, involving
    Opioid receptor agonists enhance the phosphorylation state of Fas-associated death domain (FADD) protein in the rat brain: functional interactions with casein kinase Ialpha, Galpha(i) proteins, and ERK1/2 signaling.
    Garcia-Fuster, M.J., et al.
    Neuropsychopharmacology, 55, 886-899 (2008)
    Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes.
    E Engvall et al.
    Journal of immunology (Baltimore, Md. : 1950), 109(1), 129-135 (1972-07-01)
    C K Kim et al.
    Journal of immunological methods, 159(1-2), 101-106 (1993-02-26)
    Immunoliposomes were prepared and their immunoassay applications investigated. Liposomes were prepared from cholesterol and phospholipids including maleimidobenzoylphosphatidylethanolamine (MBPE) for conjunction with thiol-containing antigens. Alkaline phosphatase (APase) was entrapped in the liposome and BSA, the antigen, was modified by reaction with
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