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Merck
CN

P2783

Phosphotransacetylase from Bacillus stearothermophilus

lyophilized powder, ≥3,000 units/mg protein (biuret)

Synonym(s):

Acetyl-CoA:orthophosphate acetyltransferase, Phosphate acetyltransferase

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About This Item

CAS Number:
9029-91-8
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-855-2
MDL number:
MFCD00131907
EC Number:
2.3.1.8 (BRENDA, IUBMB)

Key Documents

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Product Name

Phosphotransacetylase from Bacillus stearothermophilus, lyophilized powder, ≥3,000 units/mg protein (biuret)

form

lyophilized powder

specific activity

≥3,000 units/mg protein (biuret)

composition

Protein, ≥20%

shipped in

wet ice

storage temp.

−20°C

Quality Level

200

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Application

Phosphotransacetylase, from Bacillus stearothermophilus, is used to convert CoA to acetyl CoA. Phosphotransacetylase (pta) is used to study transport systems for acetate. It is used to study metabolic pathways in various bacterium.

Biochem/physiol Actions

Phosphotransacetylase converts CoA to acetyl CoA.

Other Notes

One unit will convert 1.0 μmole of CoA to acetyl CoA per min at pH 7.5 at 30 °C using acetyl phosphate as substrate.

Physical form

Lyophilized powder containing potassium phosphate

pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 3

ppe

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

常规特殊物品
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Certificates of Analysis (COA)

Lot/Batch Number
0000454533
0000454533
0000310597
0000310597
0000272087

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G D Sprott et al.
Applied and environmental microbiology, 59(4), 1092-1098 (1993-04-01)
Eleven strains of methanogenic bacteria were divided into two groups on the basis of the directionality (oxidative or reductive) of their citric acid pathways. These pathways were readily identified for most methanogens from the patterns of carbon atom labeling in
Mutants of Bacillus stearothermophilus defective in the uptake and metabolism of acetate
Philip R. Mallinder and Anne Moir
Microbiology, 137, 779-785 (1991)
Sara Castaño-Cerezo et al.
Microbial cell factories, 8, 54-54 (2009-10-27)
Acetate metabolism in Escherichia coli plays an important role in the control of the central metabolism and in bioprocess performance. The main problems related to the use of E. coli as cellular factory are i) the deficient utilization of carbon
Jing Huang et al.
Wei sheng wu xue bao = Acta microbiologica Sinica, 51(4), 480-487 (2011-07-30)
To study the effects of gene pta disruption on biosynthesis of L-tryptophan. The pta gene of the L-tryptophan producing strain E. coli TRTH was disrupted by Red recombination technology and a pta mutant E. coli TRTHdeltapta was constructed. Fed-batch fermentation
Maria Dimou et al.
Molecular biology reports, 39(4), 4135-4143 (2011-07-21)
Cyclophilins belong to the peptidyl-prolyl cis/trans isomerase family of enzymes (EC 5.2.1.8), which accelerate protein folding by catalysing the cis/trans isomerisation of proline imidic peptide bonds. In the present study, by a combination of bioinformatics methods, we identify phosphate acetyltransferase
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Articles

Coenzyme A

Enzyme Reagent Coenzyme A (CoA, CoASH or HSCoA) is the key cofactor in first step of the TCA cycle, responsible for transferring the acetyl group from pyruvate oxidation to oxaloacetate yielding citrate. Available through Sigma-Aldrich online.

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