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Safety Information

P2783

Sigma-Aldrich

Phosphotransacetylase from Bacillus stearothermophilus

lyophilized powder, ≥3,000 units/mg protein (biuret)

Synonym(s):

Acetyl-CoA:orthophosphate acetyltransferase, Phosphate acetyltransferase

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About This Item

CAS Number:
9029-91-8
EC Number:
2.3.1.8 (BRENDA, IUBMB)
EC Number:
232-855-2
MDL number:
MFCD00131907
UNSPSC Code:
12352204
NACRES:
NA.54

¥6,583.90

Please contact Customer Service for Availability
New, lower price on this item!

form

lyophilized powder

specific activity

≥3,000 units/mg protein (biuret)

composition

Protein, ≥20%

shipped in

wet ice

storage temp.

−20°C

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This Item
C2900C4899C8413
Phosphotransacetylase from Bacillus stearothermophilus lyophilized powder, ≥3,000 units/mg protein (biuret)

P2783

Phosphotransacetylase from Bacillus stearothermophilus

氯霉素乙酰转移酶 来源于大肠杆菌 lyophilized powder, 50,000-150,000 units/mg protein (Lowry)

C2900

氯霉素乙酰转移酶 来源于大肠杆菌

肉碱乙酰转移酶 来源于鸽子胸肌 ammonium sulfate suspension, ≥50 units/mg protein

C4899

肉碱乙酰转移酶 来源于鸽子胸肌

氯霉素乙酰转移酶 来源于大肠杆菌 buffered aqueous glycerol solution

C8413

氯霉素乙酰转移酶 来源于大肠杆菌

form

lyophilized powder

form

lyophilized powder

form

ammonium sulfate suspension

form

buffered aqueous glycerol solution

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

shipped in

wet ice

shipped in

dry ice

shipped in

-

shipped in

wet ice

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

-

composition

Protein, ≥20%

composition

-

composition

-

composition

-

Application

Phosphotransacetylase, from Bacillus stearothermophilus, is used to convert CoA to acetyl CoA. Phosphotransacetylase (pta) is used to study transport systems for acetate[1]. It is used to study metabolic pathways in various bacterium[2].

Biochem/physiol Actions

Phosphotransacetylase converts CoA to acetyl CoA.

Unit Definition

One unit will convert 1.0 μmole of CoA to acetyl CoA per min at pH 7.5 at 30 °C using acetyl phosphate as substrate.

Physical form

Lyophilized powder containing potassium phosphate

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H315,H319,H335

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

常规特殊物品
  • Specification Sheet

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    Certificates of Analysis (COA)

    Lot/Batch Number
    0000310597
    0000310597
    0000272087
    SLCQ3142
    0000272087

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    Mutants of Bacillus stearothermophilus defective in the uptake and metabolism of acetate
    Philip R. Mallinder and Anne Moir
    Microbiology, 137, 779-785 (1991)
    G D Sprott et al.
    Applied and environmental microbiology, 59(4), 1092-1098 (1993-04-01)
    Eleven strains of methanogenic bacteria were divided into two groups on the basis of the directionality (oxidative or reductive) of their citric acid pathways. These pathways were readily identified for most methanogens from the patterns of carbon atom labeling in
    Federico P Bologna et al.
    Journal of microbiology (Seoul, Korea), 48(5), 629-636 (2010-11-04)
    The Escherichia coli genes pta and eutD encode proteins containing the phosphate-acetyltransferase domain. EutD is composed only by this domain and belongs to the ethanolamine operon. This enzyme has not been characterized yet, and its relationship to the multimodular E.
    Guiqing Yang et al.
    Sheng wu gong cheng xue bao = Chinese journal of biotechnology, 26(2), 170-176 (2010-05-04)
    Clostridium tyrobutyricum is suitable for simultaneous saccharification and fermentation of lignocellulosic. It can produce butyric acid, acetic acid as its main fermentation products from a wide variety of carbohydrates such as glucose, xylose, cellobiose and arabinose. In order to decrease
    Maria Dimou et al.
    Molecular biology reports, 38(6), 3653-3663 (2010-11-26)
    Acetate is abundant in soil contributing to a great extent on carbon cycling in nature. Phosphate acetyltransferase (Pta, EC 2.3.1.8) catalyzes the reversible transfer of the acetyl group from acetyl-P to CoA forming acetyl-CoA and inorganic phosphate, participating to acetate
    View All Related Papers

    Articles

    Coenzyme A

    Enzyme Reagent Coenzyme A (CoA, CoASH or HSCoA) is the key cofactor in first step of the TCA cycle, responsible for transferring the acetyl group from pyruvate oxidation to oxaloacetate yielding citrate. Available through Sigma-Aldrich online.

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