C8413
氯霉素乙酰转移酶 来源于大肠杆菌
buffered aqueous glycerol solution
别名:
CAT, 乙酰辅酶 A：氯霉素 3-O-乙酰转移酶
form
buffered aqueous glycerol solution
specific activity
50,000-150,000 units/mg protein
mol wt
75 kDa (three identical subunits)
shipped in
wet ice
storage temp.
−20°C
Gene Information
Escherichia coli ... cat(2847485)
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Application
Chloramphenicol acetyltransferase from Escherichia coli has been used in a study to assess the construction of a novel expression system in Klebsiella pneumoniae and its application for 1,3-propanediol production. Chloramphenicol acetyltransferase from Escherichia coli has also been used in a study to investigate site-directed mutagenesis and promoter functional analysis of the RM07 DNA fragment from Halobacterium halobium.
The enzyme has been used in chloramphenicol acetyltransferase assay to optimize the transfection of plasmid DNA into primary cultures of adult mouse keratinocytes. It has also been used to assess the acetyl-CoA carboxylase-carboxyltransferase (ACC-CT) domain activity. This has been done using a coupled-two phase system measuring the selective partition of [14C]acetylchloramphenicol into an organic layer.
Biochem/physiol Actions
这种酶是细菌对氯霉素产生抗性的原因。催化乙酰辅酶 A 和氯霉素转化为辅酶 A 和氯霉素 3-乙酸酯。
Physical form
50% 甘油中的透明无色溶液，含 5mM Tris-HCl (pH 7.8) 和 0.5mM 2-巯基乙醇
Other Notes
在 pH 7.8 和 25°C 下，一单位本品每分钟可以将 1.0 纳摩尔氯霉素和乙酰辅酶 A 转化为氯霉素 3-乙酸酯和辅酶 A。
存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
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