生物来源
Porcine heart
质量水平
形式
ammonium sulfate suspension
比活
≥100 units/mg protein
分子量
98 kDa ( 49 kDa monomer)
溶解性
H2O: soluble 1.0 mg/mL, clear
异质活性
isocitrate dehydrogenase and aconitase ≤0.01%
malic dehydrogenase ≤0.1%
储存温度
2-8°C
应用
猪心脏来源柠檬酸合酶用于：
- 注入蝾螈卵子，确定受精时，其在卵子激活中的重要性
- 制备反应混合物，确定丙酮酸羧化酶活性
- 检查其是否能够诱导未受精卵中的[Ca22+]增加和卵子激活
生化/生理作用
柠檬酸合成酶催化柠檬酸在辅酶 A 存在下转化为乙酰辅酶 A，释放 H2O和草酰乙酸。该酶分子量为 85 kDa，pI 为 6.1-6.6。可被氟乙酰辅酶 a、棕榈酰辅酶 a 和柠檬酸辅酶 a 抑制。当其被乙酸酐乙酰化或被碘碘化时也受到抑制。
单位定义
一个装置将形成 1.0μ37°C 下 pH 8.0 时每分钟由草酰乙酸和乙酰 CoA 生成的柠檬酸盐摩尔数。
外形
用 3.2 M(NH4)2SO4 溶液 (pH 7.0) 混悬。
制备说明
溶于水，形成 1 mg/mL 浓度的澄清溶液。
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
动植物源性产品
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McDonald TS, et al.
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商品
以硫酸铵悬浮液形式提供的酶的使用指南
Instructions for working with enzymes supplied as ammonium sulfate suspensions
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