C4899
肉碱乙酰转移酶 来源于鸽子胸肌
ammonium sulfate suspension, ≥50 units/mg protein
别名:
CATC, 肉碱乙酰转移酶, CrAT, 乙酰辅酶A：肉碱-O-乙酰转移酶
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
≥50 units/mg protein
Biological source:
pigeon breast
Concentration:
≥0.4 mg/mL
biological source
pigeon breast
form
ammonium sulfate suspension
specific activity
≥50 units/mg protein
concentration
≥0.4 mg/mL
technique(s)
cell based assay: suitable
Protein ID accession no.
UniProt accession no.
storage temp.
2-8°C
Quality Level
Gene Information
pigeon ... CRAT(102084317)
General description
研究领域：细胞信号传导
Application
来自鸽子胸肌的乙酰肉碱转移酶已被用于酶测定。
Biochem/physiol Actions
乙酰肉碱转移酶（CrAT）是一种酶，有助于乙酰辅酶A和肉碱向乙酰肉碱和游离辅酶A的可逆转化。这种酶过程促进脂肪酸的β-氧化，从而在真核细胞的能量代谢中起关键作用。CrAT介导的乙酰肉碱生成和外流有助于维持线粒体中乙酰辅酶A和乙酰肉碱之间的平衡，再生游离辅酶A，并缓解丙酮酸脱氢酶（(PDH）生成的抑制。丙酮酸脱氢酶是葡萄糖氧化中的关键酶。该过程促进葡萄糖稳态，并有助于维持最佳的细胞能量代谢。乙酰肉碱转移酶活性也有助于细胞周期由G1向S期的转变。乙酰肉碱转移酶的缺乏也导致各种神经疾病的产生，包括阿尔茨海默病、共济失调性脑病和各种血管疾病。
肉碱乙酰转移酶通过催化乙酰辅酶A和乙酰-L-肉碱之间的平衡，维持细胞和线粒体水平的乙酰辅酶A，是氧化代谢所需的关键辅酶。 肉碱乙酰转移酶还维持着神经和非神经乙酰胆碱生产所需的乙酰辅酶A库。
Physical form
3.2M硫酸铵(NH4)2SO4晶体悬浮溶液，50mm磷酸钾，1mM二硫苏糖醇，pH 7.0
Analysis Note
双缩脲法测定蛋白质。
Other Notes
在pH 8.0，温度为25 °C的条件下，一单位酶每分钟可将1.0 μmole的乙酰-L-左旋肉碱和辅酶A转化为 L-左旋肉碱和乙酰辅酶A。
存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
动植物源性产品
低风险生物材料
此项目有
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商品
Instructions for working with enzymes supplied as ammonium sulfate suspensions
以硫酸铵悬浮液形式提供的酶的使用指南
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