C2900
氯霉素乙酰转移酶 来源于大肠杆菌
lyophilized powder, 50,000-150,000 units/mg protein (Lowry)
别名:
CAT, 乙酰辅酶 A：氯霉素 3-O-乙酰转移酶
产品名称
氯霉素乙酰转移酶 来源于大肠杆菌, lyophilized powder, 50,000-150,000 units/mg protein (Lowry)
form
lyophilized powder
specific activity
50,000-150,000 units/mg protein (Lowry)
mol wt
75 kDa (three identical subunits)
shipped in
dry ice
storage temp.
−20°C
Quality Level
Gene Information
Escherichia coli ... cat(2847485)
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Biochem/physiol Actions
这种酶是细菌对氯霉素产生抗性的原因。催化乙酰辅酶 A 和氯霉素转化为辅酶 A 和氯霉素 3-乙酸酯。
Other Notes
在 pH 7.8 和 25°C 下，一单位本品每分钟可以将 1.0 纳摩尔氯霉素和乙酰辅酶 A 转化为氯霉素 3-乙酸酯和辅酶 A。
Physical form
部分纯化；含 Tris 缓冲盐。
General description
The enzyme has a molecular weight of approximately 78,000 Da and pH optimum of 7.8.
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
此项目有
Detection of florfenicol resistance genes in Riemerella anatipestifer isolated from ducks and geese.
Yen-Ping Chen et al.
Veterinary microbiology, 154(3-4), 325-331 (2011-08-09)
The cat gene, coding for chloramphenicol acetyltransferase has been reported for conferring the chloramphenicol resistance for Riemerella anatipestifer. Chloramphenicol acetyltransferases, however, are unable to inactivate florfenicol. In this study, 66 R. anatipestifer isolates were investigated for their susceptibility to chloramphenicol
Y F Niu et al.
Genetics and molecular research : GMR, 10(4), 3427-3434 (2011-10-29)
Genetic transformation is useful for basic research and applied biotechnology. However, genetic transformation of microalgae is usually quite difficult due to the technical limitations of existing methods. We cloned the promoter and terminator of the nitrate reductase gene from the
Tapan Biswas et al.
Protein science : a publication of the Protein Society, 21(4), 520-530 (2012-02-02)
Novel antibiotics are needed to overcome the challenge of continually evolving bacterial resistance. This has led to a renewed interest in mechanistic studies of once popular antibiotics like chloramphenicol (CAM). Chloramphenicol acetyltransferases (CATs) are enzymes that covalently modify CAM, rendering
Hirofumi Nariya et al.
Applied and environmental microbiology, 77(23), 8439-8441 (2011-10-04)
A xylose-inducible gene expression vector for Clostridium perfringens was developed. Plasmid pXCH contains a chromosomal region from Clostridium difficile (xylR-P(xy)(lB)): xylR, encoding the xylose repressor, xylO, the xyl operator sequence, and P(xylB), the divergent promoter upstream of xylBA encoding xylulo
Junbo Liu et al.
FEBS letters, 586(12), 1719-1723 (2012-05-16)
Bicoid (Bcd) is a Drosophila morphogenetic protein and a transcriptional activator. Genetic studies have suggested a role of sumoylation in Bcd function, but it is unknown how Bcd activity is affected specifically by its own sumoylation status. Here we show
实验方案
To measure chloramphenicol acetyltransferase activity, this procedure uses DTNB and coenzyme A. The reaction of DTNB with the –SH group on CoA results in a colorimetric increase at 412 nm.
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