C2900
氯霉素乙酰转移酶 来源于大肠杆菌
lyophilized powder, 50,000-150,000 units/mg protein (Lowry)
别名:
CAT, 乙酰辅酶 A：氯霉素 3-O-乙酰转移酶
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
50,000-150,000 units/mg protein (Lowry)
form
lyophilized powder
specific activity
50,000-150,000 units/mg protein (Lowry)
mol wt
75 kDa (three identical subunits)
shipped in
dry ice
storage temp.
−20°C
Quality Level
Gene Information
Escherichia coli ... cat(2847485)
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General description
The enzyme has a molecular weight of approximately 78,000 Da and pH optimum of 7.8.
Biochem/physiol Actions
这种酶是细菌对氯霉素产生抗性的原因。催化乙酰辅酶 A 和氯霉素转化为辅酶 A 和氯霉素 3-乙酸酯。
Physical form
部分纯化；含 Tris 缓冲盐。
Other Notes
在 pH 7.8 和 25°C 下，一单位本品每分钟可以将 1.0 纳摩尔氯霉素和乙酰辅酶 A 转化为氯霉素 3-乙酸酯和辅酶 A。
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
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实验方案
To measure chloramphenicol acetyltransferase activity, this procedure uses DTNB and coenzyme A. The reaction of DTNB with the –SH group on CoA results in a colorimetric increase at 412 nm.
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