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M9281

Sigma-Aldrich

3-Methyladenine

autophagy inhibitor

Synonym(s):

3-MA, 6-Amino-3-methylpurine

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25 MG
¥1,974.51

¥1,974.51

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25 MG
¥1,974.51

About This Item

Empirical Formula (Hill Notation):
C6H7N5
CAS Number:
5142-23-4
Molecular Weight:
149.15
Beilstein:
146087
EC Number:
225-908-6
MDL number:
MFCD00010531
UNSPSC Code:
41106305
PubChem Substance ID:
24897326
NACRES:
NA.51

¥1,974.51

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biological source

synthetic (organic)

Quality Level

300

Assay

≥99% (HPLC)

form

powder

mp

~300 °C (dec.) (lit.)

solubility

DMF: 9.80-10.20 mg/mL, clear, colorless to light yellow

SMILES string

Cn1cnc(N)c2ncnc12

InChI

1S/C6H7N5/c1-11-3-10-5(7)4-6(11)9-2-8-4/h2-3H,7H2,1H3

InChI key

FSASIHFSFGAIJM-UHFFFAOYSA-N

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Show Differences

1 of 4

This Item
S7576PHR1591A7986
Buparvaquone ≥98% (HPLC)

SML1662

Buparvaquone

Shikonin ≥98% (HPLC)

S7576

Shikonin

Atovaquone Pharmaceutical Secondary Standard; Certified Reference Material

PHR1591

Atovaquone

Atovaquone ≥98% (HPLC)

A7986

Atovaquone

assay

≥98% (HPLC)

assay

≥98% (HPLC)

assay

-

assay

≥98% (HPLC)

form

powder

form

powder or solid

form

-

form

powder

Quality Level

200

Quality Level

200

Quality Level

300

Quality Level

100

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

solubility

DMSO: soluble, chloroform: soluble

solubility

methanol: 1 mg/mL

solubility

-

solubility

DMSO: >10 mg/mL

Application

3-Methyladenine (3-MA) is used to inhibit and study the mechanism of autophagy (lysosomal self-degradation) and apoptosis under various conditions. 3-MA inhibits autophagy by blocking autophagosome formation via the inhibition of type III Phosphatidylinositol 3-kinases (PI-3K). For use as an autophagy inhibitor, 3-MA is typically used at a concentration of 5 mM.
3-Methyladenine has been used as an autophagy inhibitor:
  • to determine the expressions of autophagy-related genes (Beclin 1, light chain 3 (LC3) and p62) using the transfected human bone marrow mesenchymal stem cells (BM-MSCs)[1]
  • with cisplatin to study the inhibition of autophagy influenced cisplatin-induced apoptosis in A2780cp cells[2]
  • to study the interplay between colistin-induced autophagy and apoptosis[3]

Standard for detection of nucleic acid methylation during carcinogenesis.

Biochem/physiol Actions

3-Methyladenine (3-MA) is used to inhibit and study the mechanism of autophagy (lysosomal self-degradation) and apoptosis under various conditions. 3-MA inhibits autophagy by blocking autophagosome formation via the inhibition of type III phosphatidylinositol 3-kinases (PI-3K).[4]

Preparation Note

This product is soluble in DMF (10 mg/mL; may require gentle heating). It is also soluble in water, 95% ethanol, or 1 N NaOH at 30 mg/mL with heating, but upon cooling, the product precipitates from solution. Solutions of 3-MA may be prepared in DMSO at 100 mM.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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    Certificates of Analysis (COA)

    Lot/Batch Number
    0000207629
    0000192625
    0000189657
    0000207639
    0000189657

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    Jingyuan Chen et al.
    Shock (Augusta, Ga.), 52(1), 111-121 (2018-10-05)
    Sepsis-induced myopathy is a heavy burden for patients during respiratory failure as well as after discharge, which could be characterized with qualitative changes to nAChR in a rat model of sepsis, regulated by NRG-1. Autophagy is an innate immune defense
    Heng Wang et al.
    Journal of dairy science, 102(9), 8264-8272 (2019-07-01)
    Staphylococcus aureus is an important pathogen causing chronic and subclinical mastitis of cows. Autophagy is an important regulatory mechanism that participates in the elimination of invading pathogenic organisms. Here, we hypothesize that autophagy is involved in the process of Staph.
    Ruishuang Ma et al.
    Cancer biology & therapy, 20(9), 1206-1212 (2019-05-17)
    Autophagy plays a complicated role in tumorigenesis, and the effects of autophagy in drug resistance have not been fully known. The aim of this study was to evaluate autophagy activity in lung cancer cells derived from different origins and explore
    Xiao Ye et al.
    Peptides, 119, 170120-170120 (2019-07-28)
    Insulin resistance (IR) is a fundamental pathogenic factor shared by a myriad of metabolic disorders, including obesity and type 2 diabetes. The mechanism of IR is usually accompanied by mitochondrial dysfunction. Irisin has been proposed to act as a hormone
    Do Eun Rim et al.
    Journal of biochemical and molecular toxicology, 33(8), e22348-e22348 (2019-05-09)
    Golgi S-nitro-N-acetylpenicillamine receptor complex 1 (GS28) has been implicated in Golgi vesicle transport. We examined the role of GS28 and its molecular mechanisms in sodium nitroprusside (SNP)-induced cell death using GS28 siRNA (siGS28)-transfected HeLa cells. Significant inhibition of cytotoxicity was
    View All Related Papers

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