product name
半胱天冬酶抑制剂I, Z-VAD-FMK, CAS 187389-52-2, is a cell-permeable, irreversible, pan-caspase inhibitor.
质量水平
形式
solid
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
desiccated
颜色
white
溶解性
DMSO: 50 mM
运输
ambient
储存温度
−20°C
InChI
1S/C22H30FN3O7/c1-13(2)19(26-22(31)33-12-15-8-6-5-7-9-15)21(30)24-14(3)20(29)25-16(17(27)11-23)10-18(28)32-4/h5-9,13-14,16,19H,10-12H2,1-4H3,(H,24,30)(H,25,29)(H,26,31)/t14-,16-,19-/m0/s1
InChI key
MIFGOLAMNLSLGH-QOKNQOGYSA-N
一般描述
一种细胞可渗透、不可逆的泛半胱天冬酶抑制剂。抑制Fas介导的Jurkat细胞凋亡和星形孢菌素诱导的角膜上皮细胞死亡。显示可提高人类胚胎干细胞的冻融存活率。当与纯化的天然酶或重组酶一起使用时，需要用酯酶进行预处理。
一种细胞可渗透、不可逆的泛半胱天冬酶抑制剂。显示可提高人类胚胎干细胞的冻融存活率。抑制Jurkat T细胞中Fas介导的细胞凋亡。据报道，它也抑制肽：体外和体内的N-聚糖酶 (PNGase)。当与纯化的重组酶一起使用时，需要用酯酶进行预处理。
生化/生理作用
主靶
Fas介导的Jurkat细胞凋亡和星形孢菌素诱导的角膜上皮细胞死亡
Fas介导的Jurkat细胞凋亡和星形孢菌素诱导的角膜上皮细胞死亡
产物不与ATP竞争。
可逆：否
细胞可渗透性：是
警告
毒性：标准处理（A）
序列
Z-Val-Ala-Asp(OMe)-CH₂F*
重悬
复溶后，等分并冷冻保存（-20°C）。贮备溶液可在-20°C稳定6个月。
分析说明
TLC 单点
其他说明
Heng, B.C., et al. 2007.Biosci.Rep.27, 257.
Martin, U., et al. 2007.Apoptosis12, 525.
Misaghi, S., et al. 2004.Chem. Biol.11, 1677.
Joo, C., et al. 1999.Ophthalmic Res. 31, 287.
McColl, K.S., et al. 1998.Mol.Cell.Endocrinol. 139, 229.
Thornberry, N.A. and Lazebnik, Y. 1998.Science 281, 1312.
Tolomeo, M., et al. 1998.Cell Death Differ.5, 735.
An, S. and Know, K.A. 1996.FEBS Lett.386, 115.
Cain, K., et al. 1996.Biochem J.314, 27.
Mirzoeva, O.K., et al. 1996.FEBS Lett.396, 266.
Pronk, G.J., et al. 1996.Science 271, 808.
Slee, E.A., et al. 1996.Biochem.J.315, 21.
Chow, S.C., et al. 1995.FEBS Lett.364, 134.
Fearnhead, H.O., et al. 1995.FEBS Lett.375, 283.
Zhu, H., et al. 1995.FEBS Lett.374, 303.
Martin, U., et al. 2007.Apoptosis12, 525.
Misaghi, S., et al. 2004.Chem. Biol.11, 1677.
Joo, C., et al. 1999.Ophthalmic Res. 31, 287.
McColl, K.S., et al. 1998.Mol.Cell.Endocrinol. 139, 229.
Thornberry, N.A. and Lazebnik, Y. 1998.Science 281, 1312.
Tolomeo, M., et al. 1998.Cell Death Differ.5, 735.
An, S. and Know, K.A. 1996.FEBS Lett.386, 115.
Cain, K., et al. 1996.Biochem J.314, 27.
Mirzoeva, O.K., et al. 1996.FEBS Lett.396, 266.
Pronk, G.J., et al. 1996.Science 271, 808.
Slee, E.A., et al. 1996.Biochem.J.315, 21.
Chow, S.C., et al. 1995.FEBS Lett.364, 134.
Fearnhead, H.O., et al. 1995.FEBS Lett.375, 283.
Zhu, H., et al. 1995.FEBS Lett.374, 303.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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