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GUSS

Sigma-Aldrich

β-Glucuronidase Reporter Gene Staining Kit

Synonym(s):

β-Glucuronidase Staining Kit, Reporter Gene Kit, Staining Kit for Glucuronidase

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1 KIT
¥5,501.10

¥5,501.10

Please contact Customer Service for Availability
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1 KIT
¥5,501.10

About This Item

UNSPSC Code:
12161503
NACRES:
NA.32

¥5,501.10

Please contact Customer Service for Availability

usage

 kit sufficient for 100 histochemical assays

Quality Level

200

technique(s)

microbe id | staining: suitable

shipped in

dry ice

storage temp.

−20°C

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Sigma-Aldrich

LUC1

荧光素酶报告子基因检测试剂盒

technique(s)

microbe id | staining: suitable

technique(s)

microbe id | staining: suitable

technique(s)

flow cytometry: suitable, protein staining: suitable

technique(s)

-

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

-

usage

 kit sufficient for 100 histochemical assays

usage

 kit sufficient for 100 tests (using a 3.5 cm dish)

usage

 kit sufficient for 1000 reactions (using 96 multiwell plates),  kit sufficient for 50 reactions (in a 2 mL cuvette)

usage

 kit sufficient for 100 assays

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−70°C

General description

Kit is used to anaylze β glucuronidase (E.coli GUS gene) expression in transformed plants.

Application

The E. coli GUS gene is extensively used to analyze gene expression in transformed plants. Plants have low intrinsic GUS activity, and the E. coli gene is quite stable in plant cells. As a tag, GUS remains active at the N-terminus of fusion proteins.

Kit is suitable for biochemical activity assays, immunological assays and histochemical staining of tissue and cells.

Features and Benefits

  • Histochemical staining of plant tissues expressing the E. coli GUS enzyme
  • Ideally suited to plant expression studies due to very low GUS activity in plants and high enzyme stability
  • GUS does not interfere with plant cell function or viability

Other Notes

The substrate used in this kit is X-GlcA (5-bromo-4-chloro-3-indolyl β-D-glucuronide) which results in an insoluble indigo-blue precipitate in transfected cells and tissues.

Kit Components Only

Product No.
Description
  • 2× Fixation Buffer 25 mL
  • 5-Bromo-4-chloro-3-indolyl β-D-glucuronide cyclohexylammonium salt, ≥98% 1 mL/vial
  • Reagent A for gus staining kit 50 mL
  • Reagent B for gus staining kit 200 μL
  • Reagent C for gus staining kit 200 μL

Hazard Statements

H412

Precautionary Statements

P273 - P501

Hazard Classifications

Aquatic Chronic 3

Storage Class Code

11 - Combustible Solids

  • Specification Sheet

    • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number
    0000399755
    0000399755
    0000218896
    0000218896
    0000121906

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    Find documentation for the products that you have recently purchased in the Document Library.

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    Ken Naito et al.
    Nature, 461(7267), 1130-1134 (2009-10-23)
    High-copy-number transposable elements comprise the majority of eukaryotic genomes where they are major contributors to gene and genome evolution. However, it remains unclear how a host genome can survive a rapid burst of hundreds or thousands of insertions because such
    Li-Ling Lin et al.
    International journal of molecular sciences, 14(7), 14270-14286 (2013-07-11)
    Brassinosteroids (BRs) are endogenous plant hormones and are essential for normal plant growth and development. MicroRNAs (miRNAs) of Arabidopsis thaliana are involved in mediating cell proliferation in leaves, stress tolerance, and root development. The specifics of BR mechanisms involving miRNAs
    Kuo-Hsiang Chuang et al.
    Bioconjugate chemistry, 17(3), 707-714 (2006-05-18)
    Combination therapy can help overcome limitations in the treatment of heterogeneous tumors. In the current study, we examined whether multiple therapeutic agents could be targeted to anti-dansyl single-chain antibodies (DNS scFv) that were anchored on the plasma membrane of cancer
    C-M Cheng et al.
    Cancer gene therapy, 15(6), 393-401 (2008-03-29)
    Increasing the specificity of chemotherapy may improve the efficacy of cancer treatment. Toward this aim, we developed a strain of bacteria to express enzymes for selective prodrug activation and non-invasive imaging in tumors. beta-glucuronidase and the luxCDABE gene cluster were
    R A Jefferson
    Nature, 342(6251), 837-838 (1989-12-14)
    The GUS reporter gene system is already a powerful tool for the assessment of gene activity in transgenic plants. Further developments may lead to routine in vivo analysis and fusion genetics.
    View All Related Papers

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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