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71736

Sigma-Aldrich

Sodium dodecyl sulfate solution

BioUltra, for molecular biology, 10% in H2O

Synonym(s):

Lauryl sulfate sodium salt, SDS, Sodium lauryl sulfate solution

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About This Item

Linear Formula:
CH3(CH2)11OSO3Na
CAS Number:
151-21-3
Molecular Weight:
288.38
Beilstein:
3599286
MDL number:
MFCD00036175
UNSPSC Code:
12161902
eCl@ss:
39093306
PubChem Substance ID:
57652355
NACRES:
NA.26

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Molecular Biology
for molecular biology

description

anionic

product line

BioUltra

form

liquid

mol wt

288.38 g/mol

concentration

10% in H2O

technique(s)

HPLC: suitable
protein quantification: suitable

impurities

DNases, none detected
 RNases, none detected
 insoluble matter, passes filter test
 phosphatases, none detected
 proteases, none detected

pH

5.0-6.5

density

1.01 g/mL at 20 °C

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biological source

mouse

biological source

rabbit

biological source

mouse

biological source

mouse

Gene Information

human ... GFAP(2670)

Gene Information

human ... GFAP(2670)

Gene Information

human ... GFAP(2670)

Gene Information

human ... GFAP(2670)

clone

GA5, monoclonal

clone

polyclonal

clone

GA5, monoclonal

clone

GA5, monoclonal

antibody form

purified immunoglobulin

antibody form

serum

antibody form

ascites fluid

antibody form

purified immunoglobulin

isotype

IgG1

isotype

-

isotype

IgG1

isotype

IgG1κ

Application

Sodium dodecyl sulfate solution has been used:
  • in the preparation of 2x extraction buffer[1]
  • as a component in elution buffer for immunoprecipitation[2]
  • in SDS–polyacrylamide gel electrophoresis (PAGE) analysis[3][4]

Biochem/physiol Actions

Sodium dodecyl sulfate (SDS)/lauryl sulfate, is an ionic detergent has the ability to disrupt biological membranes. It can block ribonuclease (RNase) and deoxyribonuclease (DNase) activity. SDS is one of the main constituents of several reagents, that are used to purify nucleic acids.[5] It is also considered as a permeability enhancer in oral formulations.[6]

Other Notes

In large-scale phenol extraction of RNA - promotes the dissociation of protein from nucleic acids when extracting from biological material[7]

also commonly purchased with this product

Product No.
Description
Pricing

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H315,H319

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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    Certificates of Analysis (COA)

    Lot/Batch Number
    BCCM9637
    BCCM3869
    BCCL6561
    BCCL3786
    BCCM4107

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    Obtaining information about protein secondary structures in aqueous solution using Fourier transform IR spectroscopy
    Yang H, et al.
    Nature Protocols, 10(3), 382-382 (2015)
    D.M. Wallace
    Methods in Enzymology, 125, 39-39 (1987)
    Porous nucleating agents for protein crystallization
    Khurshid S, et al.
    Nature Protocols, 9(7), 1621-1621 (2014)
    Dictyostelium cultivation, transfection, microscopy and fractionation
    Hirst J, et al.
    Bio-protocol, 5(11) (2015)
    Construction of FWPV Chimaeric MVA
    Buttigieg K R and Skinner M A
    Bio-protocol, 5(1) (2015)
    View All Related Papers

    Protocols

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