LC/MS Analysis of Melamine and Hydrolysis Products on Ascentis^® Express HILIC

column

Ascentis Express HILIC, 5 cm x 2.1 mm I.D., 2.7 μm particles (53934-U)

mobile phase

5 mM ammonium formate in 95:5 (v/v) acetonitrile:water

flow rate

0.2 mL/min

column temp.

35 °C

detector

MS, ESI(+), full scan

injection

2 μL

sample

1 mg/L in mobile phase