跳转至内容
Merck
CN

HPLC Analysis of Vitamin D2 and Vitamin D3 on Ascentis® C18

HPLC Analysis of Vitamin D2 and Vitamin D3 on Ascentis® C18 application for HPLC

材料

分析色谱柱

产品编号
说明
价格

Ascentis ® C18 HPLC 色谱柱

5 μm particle size, L × I.D. 15 cm × 4.6 mm

标准

产品编号
说明
价格

胆钙化固醇(维生素 D3)

analytical standard

麦角钙化甾醇 (D2)

analytical standard

CONDITIONS

column

Ascentis C18, 15 cm x 4.6 mm I.D., 5 μm particles (581324-U)

mobile phase

acetonitrile

flow rate

1.0 mL/min

column temp.

35 °C

detector

UV, 290 nm

sample

50 μg/mL each in acetonitrile

说明

分析说明

This application demonstrates the suitability of the Ascentis C18 for the analysis of the fat-soluble vitamins D2 and D3 . Structures along with the optimized chromatogram obtained on the Ascentis C18 are presented.

法律信息

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany