HPLC Analysis of Vitamin D2 and Vitamin D3 on Ascentis® C18
CONDITIONS
column
Ascentis C18, 15 cm x 4.6 mm I.D., 5 μm particles (581324-U)
mobile phase
acetonitrile
flow rate
1.0 mL/min
column temp.
35 °C
detector
UV, 290 nm
sample
50 μg/mL each in acetonitrile
说明
分析说明
This application demonstrates the suitability of the Ascentis C18 for the analysis of the fat-soluble vitamins D2 and D3 . Structures along with the optimized chromatogram obtained on the Ascentis C18 are presented.
法律信息
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany