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HPLC Analysis of Sugars and Taurine in an Energy Drink on Ascentis® Express HILIC using UV and ELSD Detection in Series

HPLC Analysis of Sugars and Taurine in an Energy Drink on Ascentis® Express HILIC using UV and ELSD Detection in Series application for HPLC

材料

分析色谱柱

产品编号
说明
价格

53970-U

Ascentis® Express HILIC, 2.7 μm HPLC色谱柱

2.7 μm particle size, L × I.D. 10 cm × 3 mm

标准

产品编号
说明
价格

S9378

蔗糖

≥99.5% (GC)

T0625

牛磺酸

≥99%

1286504

果糖

United States Pharmacopeia (USP) Reference Standard

G0350500

D -(+)-葡萄糖

European Pharmacopoeia (EP) Reference Standard

CONDITIONS

column

Ascentis Express HILlC, 10 cm x 3.0 mm I.D., 2.7 μm particles (53970-U)

mobile phase

[A] 100 mM ammonium acetate, pH 5.0 with acetic acid; [B] water; [C] acetonitrile; (09:01:90, A:B:C)

flow rate

0.6 mL/min

pressure

812 psi (56 bar)

column temp.

35 °C

detector

ELSD, 55 °C, 3.5 bar nitrogen

injection

2 μL

sample

dilute1:9 in acetonitrile

说明

分析说明

This application demonstrates the suitability of Ascentis Express HILIC for the efficient separation of common drink additives used in caffeinated energy drinks. The analysis was performed with UV and ELSD detectors in series, this chromatogram being from the ELSD.

法律信息

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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