HPLC Analysis of Oligonucleotides on Proteomix^® SAX

column

Proteomix SAX-NP5, 25 cm x 4.6 mm I.D., 5 μm particles (Z777229)

mobile phase

A: 20 mM Tris, pH 8.0 B: A + 0.5 M NaCl

gradient

0 - 100% B in 30 minutes

flow rate

0.5 mL/min

pressure

1073 psi (74 bar)

column temp.

25 °C

detector

UV 260 nm

injection

5 μL

sample

(10 mM each oligonucleotide in water)