HPLC Analysis of Hydrocortisone in Topical Cream on Discovery® HS C18 after SPE using Discovery® DSC-Si
CONDITIONS
sample preparation
SPE (Solid Phase Extraction)
sample addition
hydrocortisone topical hand cream in hexane:ethylacetate
SPE tube/cartridge
Discovery DSC-Si, 500 mg/3 mL (52695-U)
condition
1 mL hexane:ethyl acetate (2:1, v/v)
sample addition
1 mL
washing
1 mL hexane:ethyl acetate (2:1, v/v)
elution
1 mL methanol:DI water (1:1, v/v)
eluate post-treatment
dilute eluate to 2 mL with methanol:DI water (1:1, v/v)
column
Discovery HS C18, 15 cm x 4.6 mm I.D., 5 μm particles (568520-U)
mobile phase
[A] methanol; [B] DI water (1:1, A:B)
flow rate
1 mL/min
column temp.
35 °C
detector
UV, 220 nm
injection
10 μL
说明
应用
Sample Pre-treatment:
Dissove 1 g 1% hydrocortisone topical hand cream in 10 mL ethyl acetate. Dilute 2 mL of ethyl acetate-cream sample with 8 mL hexane:ethyl acetate (2:1, v/v).
法律信息
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany