HPLC Analysis of Flurbiprofen Enantiomers on Astec^® Cellulose DMP

column

Astec Cellulose DMP, 15 cm x 4.6 mm I.D., 5 μm particles (51098AST)

mobile phase

[A] heptane; [B] isopropanol; [C] trifluoroacetic acid (95:5:0.1, A:B:C)

flow rate

0.5 mL/min

column temp.

25 °C

detector

UV, 230 nm

injection

3 μL

sample

1 g/L in methanol