HPLC Analysis of Fab/Fc mAb Antibody Fragments on Proteomix^® WCX NP1.7

column

Proteomix WCX-NP1.7, 3 cm x 4.6 mm I.D., 1.7 μm particles (Z777179)

mobile phase

(A) 20 mM sodium acetate, pH 5.15; (B) A + 1 M LiCl

gradient

12% to 40% B in 6.7 min; to 100% B in 0.1 min; held for 2 min

flow rate

(1.5 mL/min)

pressure

3335-3916 psi (230 - 270 bar)

detector

UV 280 nm

injection

25 μL

sample

1 mg/mL papain digested MAb 321