HPLC Analysis of E. coli Lysate on Proteomix® SAX Columns, Particle Size Comparison
CONDITIONS
column temp.
25 °C
column
Proteomix SAX-NP10, 5 cm x 4.6 mm I.D., 10 μm particles (Z777203)
column
Proteomix SAX-NP5, 5 cm x 4.6mm I.D., 5 μm particles (Z777227)
detector
UV, 280 nm
flow rate
0.5 mL/min
gradient
0 - 100%B in 30 min
injection
10 μL
mobile phase
(A) 20 mM Tris, pH 9.0; (B) A + 0.5 M NaCl
sample
2.5 mg/mL
说明
法律信息
Proteomix is a registered trademark of Sepax Technologies