HPLC Analysis of Deprenyl (Selegiline) Enantiomers on Astec^® CYCLOBOND^™ I 2000 DMP

column

CYCLOBOND I 2000 DMP, 25 cm x 4.6 mm I.D., 5 μm particles (20724AST)

mobile phase

[A] 20 mM ammonium acetate, pH 4.0; [B] methanol; (65:35, A:B)

flow rate

1 mL/min

column temp.

25 °C

detector

UV, 254 nm

injection

10 μL

sample

1 mg/mL in methanol