HPLC Analysis of a Monoclonal Antibody on Zenix^® SEC-300 after Partial Proteolysis with IdeS Protease

材料

07298

FabRICATOR^®

for cleaving 2 mg IgG, 2000 U/vial

CONDITIONS

column

Zenix SEC-300, 30 cm X 4.6 mm I.D., 3 μm particles (Z777087)

mobile phase

0.15 M potassium phosphate monobasic, pH 7.0 with potassium hydroxide

flow rate

0.35 mL/min

pressure

1350 psi (93 bar)

column temp.

ambient

detector

UV, 215 nm

injection

1 μL

sample

digested SO57 mAb, ~0.5 g/L

说明

分析说明

Proteolysis of antibodies can be useful for simplifying further analysis. This application demonstrates separation of the two fragments, F(ab′)2 and scFc from the intact IgG, after partial digestion with IdeS protease.

法律信息

Zenix is a registered trademark of Sepax Technologies

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Small Molecule HPLC Large Molecule HPLC Gas Chromatography (GC)TLC Solid Phase Microextraction (SPME)Solid Phase Extraction (SPE)QuEChERS

HPLC Analysis of a Monoclonal Antibody on Zenix® SEC-300 after Partial Proteolysis with IdeS Protease

材料

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