HPLC Analysis of a Monoclonal Antibody on Zenix^® SEC-150, 30 cm X 4.6 mm I.D., 3 μm particles after Proteolysis with IdeS Protease

材料

07298

FabRICATOR^®

for cleaving 2 mg IgG, 2000 U/vial

CONDITIONS

column

Zenix^® SEC-150, 30 cm X 4.6 mm I.D., 3 μm particles (Z777018)

mobile phase

0.15 M potassium phosphate monobasic, pH 7.0 with potassium hydroxide

flow rate

0.35 mL/min

pressure

1450 psi (100 bar)

column temp.

ambient

detector

UV, 215 nm

injection

1 μL

sample

digested SO57 mAb, ~0.5 g/L

说明

分析说明

Proteolysis of antibodies can be useful for simplifying further analysis. This application demonstrates separation of the two fragments, F(ab′)2 and scFc, after digestion with IdeS protease

法律信息

Zenix is a registered trademark of Sepax Technologies

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HPLC Columns GC Columns & Accessories TLC Plates & Adsorbents Solid Phase Microextraction (SPME)Solid Phase Extraction & QuEChERS

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Small Molecule HPLC Large Molecule HPLC Gas Chromatography (GC)TLC Solid Phase Microextraction (SPME)Solid Phase Extraction (SPE)QuEChERS

HPLC Analysis of a Monoclonal Antibody on Zenix® SEC-150, 30 cm X 4.6 mm I.D., 3 μm particles after Proteolysis with IdeS Protease

材料

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