HPLC Analysis of 3,5-DNB-Tryptophan Methyl Ester Enantiomers on Astec^® CYCLOBOND I 2000 SN

column

CYCLOBOND I 2000 SN, 25 cm x 4.6 mm I.D., 5 μm particles (20524AST)

mobile phase

ethanol

flow rate

1 mL/min

column temp.

25 °C

detector

UV, 230 nm

injection

5 μL

sample

1 mg/mL in methanol