GC Analysis of Volatiles in Milk Thistle Honey on SUPELCOWAX^™ 10 after SPME using 50/30 μm DVB/Carboxen/PDMS Fiber

sample/matrix

5 g milk thistle honey + 5 mL bidistilled water in a 20 mL vial, allowed to equilibrate at 50 °C for 15 min with magnetic stirring

SPME fiber

2 cm 50/30 μm divinylbenzene/carboxen on polydimethylsiloxane (57348-U)

extraction

headspace, 50 °C for 40 min with magnetic stirring

desorption process

250 °C for 2 min

column

SUPELCOWAX 10, 30 m x 0.25 mm I.D., 0.25 μm (24079)

oven

35 °C (8 min), 6 °C/min to 60 °C, 4 °C/min to 160 °C, 20 °C/min to 200 °C (1 min)

MSD interface

230 °C

scan range

35-300 m/z

carrier gas

helium, 1 mL/min

liner

0.75 mm I.D., SPME