GC Analysis of Pesticides in Avocado on SLB^®-5ms (20 m x 0.18 mm I.D., 0.36 μm) after QuEChERS Cleanup using Supel^™ QuE Z-Sep+, Fast GC Analysis

sample preparation

Dispersive (QuEChERS)

sample/matrix

Avocado spiked with pesticides at 20 ng/g

extraction tube

Supel QuE Acetate extraction tube (55234-U)

extraction process

add 25 mL of acetonitrile; shake for 1 minute; add contents of Supel QuE acetate extraction tube; shake immediately for 1 minute; centrifuge at 3200 rpm for 5 minutes

clean-up tube

Supel Que Z-Sep+ cleanup tube (55296-U)

clean-up process

transfer 3 mL of the acetonitrile layer into a Supel Que Z-Sep+ cleanup tube; shake for 1 minute; centrifuge at 3400 rpm for 3 minutes; draw off supernatant for LVI-GCMS analysis

column

SLB-5ms, 20 m x 0.18 mm I.D., 0.36 μm (28576-U)

oven

70 °C (2 min), 15 °C/min to 325 °C (6 min)

inj. temp.

60 °C (0.28 min), 600 °C/min to 325 °C (5 min)

detector

MSD

MSD interface

330 °C

carrier gas

helium, 1 mL/min, constant

injection

PTV solvent vent; 10 μL, injection dispense speed 15000 μL/min

liner

4 mm I.D. FocusLiner with taper

sample

pesticides spiked at 20 ng/g