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  • Polar lipids from the marine macroalga Palmaria palmata inhibit lipopolysaccharide-induced nitric oxide production in RAW264.7 macrophage cells.

Polar lipids from the marine macroalga Palmaria palmata inhibit lipopolysaccharide-induced nitric oxide production in RAW264.7 macrophage cells.

Phytochemistry (2014-02-27)
Arjun H Banskota, Roumiana Stefanova, Sandra Sperker, Santosh P Lall, James S Craigie, Jeff T Hafting, Alan T Critchley
摘要

The EtOAc soluble fraction of a MeOH/CHCl3 extract of Palmaria palmata showed strong nitric oxide (NO) inhibitory activity against lipopolysaccharide (LPS)-induced NO production in murine RAW264.7 cells. NO inhibition-guided isolation led to identification of three new polar lipids including a sulfoquinovosyl diacylglycerol (SQDG) (2S)-1-O-eicosapentaenoyl-2-O-myristoyl-3-O-(6-sulfo-α-D-quinovopyranosyl)-glycerol (1) and two phosphatidylglycerols, 1-O-eicosapentaenoyl-2-O-trans-3-hexadecenoyl-3-phospho-(1'-glycerol)-glycerol (3) and 1-O-eicosapentaenoyl-2-O-palmitoyl-3-phospho-(1'-glycerol)-glycerol (4) from the EtOAc fraction. Seven known lipids were also isolated including a SQDG (2), a phospholipid (5) and five galactolipids (6-10). Structures of the isolated lipids were elucidated by spectral analyses. The isolated SQDGs, phosphatidylglycerols and phospholipid possessed strong and dose-dependent NO inhibitory activity compared to N(G)-methyl-L-arginine acetate salt (L-NMMA), a well-known NO inhibitor used as a positive control. Further study suggested that these polar lipids suppressed NO production through down-regulation of inducible nitric oxide synthase (iNOS).

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Sigma-Aldrich
亚硝酸钠, ACS reagent, ≥97.0%
Sigma-Aldrich
亚硝酸钠, ReagentPlus®, ≥99.0%
Sigma-Aldrich
亚硝酸钠, 99.999% trace metals basis
Supelco
亚硝酸钠, analytical standard
Supelco
亚硝酸根离子标准溶液, 0.1 M NO2-, for ion-selective electrodes
Sigma-Aldrich
亚硝酸钠, anhydrous, Redi-Dri, ReagentPlus®, ≥99.0%
Sigma-Aldrich
亚硝酸钠, anhydrous, free-flowing, Redi-Dri, ACS reagent, ≥97%
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亚硝酸钠, puriss., meets analytical specification of Ph. Eur., BP, USP, FCC, E 250, 99-100.5% (calc. to the dried substance)