In situ activation of bis-dialkylaminophosphines--a new method for synthesizing deoxyoligonucleotides on polymer supports.

In situ activation of bis-dialkylaminophosphines--a new method for synthesizing deoxyoligonucleotides on polymer supports.

Nucleic acids research (1984-05-25)

A D Barone, J Y Tang, M H Caruthers

摘要

Deoxynucleoside phosphoramidites can be prepared in good yield from deoxynucleosides, bis- dialkylaminophosphines , and the corresponding dialkylamine hydrotetrazolide or tetrazole as catalysts. These phosphoramidites generated in situ lead to the direct synthesis of deoxyoligonucleotides on polymer supports.

材料

货号

品牌

产品描述

88185

Sigma-Aldrich

四唑溶液, suitable for DNA synthesis, filtered through a 1 μm filter, ~0.45 M in acetonitrile