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Merck
CN
  • A simple high-performance liquid chromatography method for simultaneous determination of three triazole antifungals in human plasma.

A simple high-performance liquid chromatography method for simultaneous determination of three triazole antifungals in human plasma.

Antimicrobial agents and chemotherapy (2012-11-14)
Mei Zhang, Grant A Moore, Murray L Barclay, Evan J Begg
摘要

A rapid and simple high-performance liquid chromatography (HPLC) assay was developed for the simultaneous determination of three triazole antifungals (voriconazole, posaconazole, and itraconazole and the metabolite of itraconazole, hydroxyitraconazole) in human plasma. Sample preparation involved a simple one-step protein precipitation with 1.0 M perchloric acid and methanol. After centrifugation, the supernatant was injected directly into the HPLC system. Voriconazole, posaconazole, itraconazole, its metabolite hydroxyitraconazole, and the internal standard naproxen were resolved on a C(6)-phenyl column using gradient elution of 0.01 M phosphate buffer, pH 3.5, and acetonitrile and detected with UV detection at 262 nm. Standard curves were linear over the concentration range of 0.05 to 10 mg/liter (r(2) > 0.99). Bias was <8.0% from 0.05 to 10 mg/liter, intra- and interday coefficients of variation (imprecision) were <10%, and the limit of quantification was 0.05 mg/liter.

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(S)-(+)-6-甲氧基-α-甲基-2-萘乙酸, 98%
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萘普生, Pharmaceutical Secondary Standard; Certified Reference Material
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Supelco
萘普生 溶液, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®
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萘普生, meets USP testing specifications
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萘普生, European Pharmacopoeia (EP) Reference Standard
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Supelco
氧萘丙酸, VETRANAL®, analytical standard
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5 mg
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25 mg
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¥6,250.70