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  • Enhancement of invertase production by Aspergillus niger OZ-3 using low-intensity static magnetic fields.

Enhancement of invertase production by Aspergillus niger OZ-3 using low-intensity static magnetic fields.

Preparative biochemistry & biotechnology (2013-01-11)
Mesut Taskin, Nevzat Esim, Mucip Genisel, Serkan Ortucu, Ismet Hasenekoglu, Ozden Canli, Serkan Erdal
摘要

The aim of this study is to investigate the effect of low-intensity static magnetic fields (SMFs) on invertase activity and growth on different newly identified molds. The most positive effect of SMFs on invertase activity and growth was observed for Aspergillus niger OZ-3. The submerged production of invertase was performed with the spores obtained at the different exposure times (120, 144, 168, and 196 hr) and magnetic field intensities (0.45, 3, 5, 7, and 9 mT). The normal magnetic field of the laboratory was assayed as 0.45 mT (control). Optimization of magnetic field intensity and exposure time significantly increased biomass production and invertase activity compared to 0.45 mT. The maximum invertase activity (51.14 U/mL) and biomass concentration (4.36 g/L) were achieved with the spores obtained at the 144 hr exposure time and 5 mT magnetic field intensity. The effect of low-intensity static magnetic fields (SMFs) on invertase activities of molds was investigated for the first time in the present study. As an additional contribution, a new hyper-invertase-producing mold strain was isolated.

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Sigma-Aldrich
转化酶 来源于面包酵母(酿酒酵母), Grade VII, ≥300 units/mg solid
Sigma-Aldrich
转化酶 来源于产朊假丝酵母, Grade X, ≥300 units/mg solid
Supelco
转化酶 来源于面包酵母(酿酒酵母), 200-300 units/mg solid
Sigma-Aldrich
转化酶 来源于面包酵母(酿酒酵母), BioReagent, from Saccharomyces cerevisiae, for proteomics