Protein complexes including PGRMC1 and actin-associated proteins are disrupted by AG-205.

Protein complexes including PGRMC1 and actin-associated proteins are disrupted by AG-205.

Biochemical and biophysical research communications (2020-01-26)

Sarah L Teakel, Marina Ludescher, Bashar M Thejer, Gereon Poschmann, Jade K Forwood, Hans Neubauer, Michael A Cahill

PMID31980178

摘要

PGRMC1 is a protein from the MAPR family with a range of cellular functions. PGRMC1 has been described to play a role in fertility, neuroprotection, steroidogenesis, membrane trafficking and in cancer cell biology. PGRMC1 represents a likely key regulator of cell metabolism and proliferation, as well as a potential target for anti-cancer therapies. To further understand the functions of PGRMC1 and the mechanism of the small molecule inhibitor of PGRMC1, AG-205, proteins differentially bound to PGRMC1 were identified following AG-205 treatment of MIA PaCa-2 cells. Our results suggest that AG-205 influences PGRMC1 interactions with the actin cytoskeleton. The binding of two PGRMC1-associated proteins that support this, RACK1 and alpha-Actinin-1, was reduced following AG-205 treatment. The biology associated with PGRMC1 binding partners identified here merits further investigation.

材料

货号

品牌

产品描述

P8340

Sigma-Aldrich

Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution

T0565

Sigma-Aldrich

用于膜的3,3′,5,5′-四甲基联苯胺（TMB）液体底物系统, ready to use solution

X100

Sigma-Aldrich

Triton^™ X-100, laboratory grade

C1992

Sigma-Aldrich

ColorBurst ^™ 电泳标记, mol wt 8,000-220,000 Da

400052

Millipore

潮霉素B，来源于链霉菌属，无菌过滤溶液，溶剂为PBS，经细胞培养测试, Hygromycin B is an aminoglycoside antibiotic that inhibits the growth of prokaryotic and eukaryotic microorganisms and mammalian cells. Inhibits protein synthesis by interfering with the translocation of the 70S ribosome and causes misreading of mRNA.