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Merck
CN

V900503

Sigma-Aldrich

过氧化物酶 来源于辣根

Vetec, reagent grade

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别名:
供体:过氧化氢氧化还原酶, 辣根过氧化物酶
CAS号:
EC 号:
MDL编号:
UNSPSC代码:
12352204

等级

reagent grade

产品线

Vetec

形式

powder

比活

≥250 units/mg solid

分子量

~44 kDa

储存温度

2-8°C

InChI

1S/H2O3/c1-3-2/h1-2H

InChI key

JSPLKZUTYZBBKA-UHFFFAOYSA-N

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相关类别

一般描述

辣根过氧化物酶是从辣根(Amoracia rusticana)中分离出来的,属于过氧化物酶的铁原卟啉基团。HRP是含有四个二硫键的单链多肽。它是一种含有18%碳水化合物的糖蛋白。碳水化合物由半乳糖、阿拉伯糖、木糖、岩藻糖、甘露糖、甘露糖胺和半乳糖胺组成,具体取决于特定同工酶。其分子量(约44 kDa)包括多肽链(33,890道尔顿)、氯化血红素加Ca2+ (约700道尔顿)和碳水化合物(约9,400道尔顿)。至少存在7种HRP同工酶。 辣根过氧化物酶同工酶的等电点范围为3.0-9.0。

单位定义

一个连苯三酚单元将在20℃、pH 6.0条件下,在20秒内从连苯三酚形成1.0mg的红棓酚。

分析说明

RZ(Reinheitszahl)是指溶于去离子水中的酶(0.5–1.0 mg/ml)的吸光度比值A403/A275 。 它是血红素含量的量度,而不是酶活性。 即使是RZ值较高的制剂也可能具有较低的酶活性。

其他说明

有关过氧化物酶的更多信息,请访问 www.sigma-aldrich.com/enzymeexplorer

法律信息

Vetec is a trademark of Merck KGaA, Darmstadt, Germany

象形图

Health hazard

警示用语:

Danger

危险声明

危险分类

Resp. Sens. 1 - Skin Sens. 1

WGK

WGK 3


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