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Merck
CN

V900412

三(羟甲基)甲基甘氨酸

Vetec, reagent grade, ≥99%

别名:

N-三(羟甲基)甲基甘氨酸

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关于此项目

线性分子式:
(HOCH2)3CNHCH2CO2H
化学文摘社编号:
分子量:
179.17
EC Number:
227-193-6
UNSPSC Code:
12161700
PubChem Substance ID:
Beilstein/REAXYS Number:
1937804
MDL number:
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grade

reagent grade

product line

Vetec

assay

≥99%

form

crystalline powder

useful pH range

7.4-8.8

pKa (25 °C)

8.1

SMILES string

OCC(CO)(CO)NCC(O)=O

InChI

1S/C6H13NO5/c8-2-6(3-9,4-10)7-1-5(11)12/h7-10H,1-4H2,(H,11,12)

InChI key

SEQKRHFRPICQDD-UHFFFAOYSA-N

General description

三(羟甲基)甲基甘氨酸是最常用的电泳缓冲液,其负电荷比甘氨酸低。这反过来又有助于它更快地迁移。三(羟甲基)甲基甘氨酸也用于细胞团块的再悬浮。此外,它的高离子强度导致更多的离子运动和更少的蛋白质运动。这有助于在较低百分比的丙烯酰胺凝胶中分离低分子量蛋白质。与甘氨酸-十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)系统相比,三(羟甲基)甲基甘氨酸也被用作拖尾离子,有助于在较低丙烯酰胺浓度下解析小蛋白质。

Application

用于分离低分子量的肽的缓冲剂组分。

Legal Information

Vetec is a trademark of Merck KGaA, Darmstadt, Germany


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存储类别

13 - Non Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



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Lot/Batch Number

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Encyclopedia of Dairy Sciences (2011)
Thierry Rabilloud
Journal of proteomics, 73(8), 1562-1572 (2010-04-17)
Electrophoretic separations of proteins are widely used in proteomic analyses, and rely heavily on SDS electrophoresis. This mode of separation is almost exclusively used when a single dimension separation is performed, and generally represents the second dimension of two-dimensional separations.
Arpita Gantayet et al.
Biofouling, 29(1), 77-85 (2012-12-06)
The freshwater zebra mussel (Dreissena polymorpha) is a notorious biofouling organism. It adheres to a variety of substrata underwater by means of a proteinaceous structure called the byssus, which consists of a number of threads with adhesive plaques at the



全球贸易项目编号

货号GTIN
V900412-100G04061833469927
V900412-500G04061833495032