推荐产品
等级
reagent grade
产品线
Vetec™
方案
≥99%
表单
crystalline powder
有效pH范围
7.4-8.8
pKa (25 °C)
8.1
SMILES字符串
OCC(CO)(CO)NCC(O)=O
InChI
1S/C6H13NO5/c8-2-6(3-9,4-10)7-1-5(11)12/h7-10H,1-4H2,(H,11,12)
InChI key
SEQKRHFRPICQDD-UHFFFAOYSA-N
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一般描述
三（羟甲基）甲基甘氨酸是最常用的电泳缓冲液，其负电荷比甘氨酸低。这反过来又有助于它更快地迁移。三（羟甲基）甲基甘氨酸也用于细胞团块的再悬浮。此外，它的高离子强度导致更多的离子运动和更少的蛋白质运动。这有助于在较低百分比的丙烯酰胺凝胶中分离低分子量蛋白质。与甘氨酸-十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）系统相比，三（羟甲基）甲基甘氨酸也被用作拖尾离子，有助于在较低丙烯酰胺浓度下解析小蛋白质。
应用
用于分离低分子量的肽的缓冲剂组分。
法律信息
Vetec is a trademark of Merck KGaA, Darmstadt, Germany
储存分类代码
13 - Non Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Encyclopedia of Dairy Sciences (2011)
Arpita Gantayet et al.
Biofouling, 29(1), 77-85 (2012-12-06)
The freshwater zebra mussel (Dreissena polymorpha) is a notorious biofouling organism. It adheres to a variety of substrata underwater by means of a proteinaceous structure called the byssus, which consists of a number of threads with adhesive plaques at the
Christian Nilsson et al.
Electrophoresis, 31(3), 459-464 (2010-02-02)
Totally porous lipid-based liquid crystalline nanoparticles were used as pseudostationary phase for capillary electroseparation with LIF detection of proteins at physiological conditions using unmodified cyclic olefin copolymer capillaries (Topas, 6.7 cm effective length). In the absence of nanoparticles, i.e. in
Thierry Rabilloud
Journal of proteomics, 73(8), 1562-1572 (2010-04-17)
Electrophoretic separations of proteins are widely used in proteomic analyses, and rely heavily on SDS electrophoresis. This mode of separation is almost exclusively used when a single dimension separation is performed, and generally represents the second dimension of two-dimensional separations.
Hermann Schägger
Nature protocols, 1(1), 16-22 (2007-04-05)
Tricine-SDS-PAGE is commonly used to separate proteins in the mass range 1-100 kDa. It is the preferred electrophoretic system for the resolution of proteins smaller than 30 kDa. The concentrations of acrylamide used in the gels are lower than in
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