等级
pharmaceutical primary standard
制造商/商品名称
USP
应用
pharmaceutical
格式
neat
储存温度
−70°C
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一般描述
胶原蛋白酶II是一种金属蛋白酶，起内肽酶作用，并表现出三肽羧肽酶活性。显示内肽酶活性时，主要裂解位点位于人类胶原蛋白双链氨基酸甘氨酸-脯氨酸之前。水解发生在胶原蛋白三螺旋区域的内部。胶原蛋白酶II也被称为II类胶原蛋白酶，包含三种亚型：δ、ε和ζ。 胶原蛋白酶II USP参考标准按现行药典规定交付。所有为支持本产品而提供的信息，包括MSDS和任何产品信息单均由药典颁发机构制定并发布。如需进一步信息和支持，请访问现行药典网站。
应用
- Collagenase Type II for Enhanced Cell Isolation in Biomedical Research: Collagenase Type II is extensively applied in tissue dissociation for isolating cells, crucial for regenerative medicine and tissue engineering. It enables efficient breakdown of the extracellular matrix, facilitating easier cell extraction and purification for use in 3D cell culture and stem cell research. This enzymatic method is invaluable for studies requiring high-purity cell populations, making it indispensable in developing therapies for diseases like Parkinson′s and Alzheimer′s (Park et al., 2024).
- Advancing Tissue Engineering with Collagenase II: In tissue engineering, Collagenase II plays a pivotal role by degrading the collagen matrix in tissues, thereby enabling the cultivation of cells in a controlled environment. This process is essential for constructing tissue structures from the ground up, particularly in developing organoids that mimic natural organ functionalities. The use of high-grade biotech collagenase ensures reproducibility and effectiveness in these sophisticated applications (Wu et al., 2024).
- Collagenase II in 3D Cell Culture Systems: Collagenase II is instrumental in preparing extracellular matrices for 3D cell cultures, which are critical for studying cell behaviors in environments that closely replicate in vivo conditions. This enzyme helps in the accurate study of cellular interactions and pharmacological responses, providing a more realistic assessment of cellular dynamics in medical research (Jiang et al., 2024).
- Role of Collagenase II in Stem Cell Research: Utilizing Collagenase II in stem cell research enhances the isolation and maintenance of stem cells. This is particularly important for applications where the integrity and viability of stem cells are crucial, such as in regenerative medicine and therapeutic cloning. The enzyme′s ability to gently dissociate cells while preserving their functionality makes it a staple in cutting-edge biomedical research (Simón-Pérez et al., 2024).
- Collagenase II for Effective Extracellular Matrix Degradation: The ability of Collagenase II to efficiently degrade collagen-rich extracellular matrices is utilized in various biomedical applications, including cancer research where it aids in tumor microenvironment studies and drug penetration analyses. This enzymatic activity is crucial for understanding the interactions between cancer cells and their surrounding stroma, providing insights into potential therapeutic targets (Mohamed et al., 2024).
生化/生理作用
每摩尔胶原蛋白酶被4g原子钙活化。 它被乙二醇-双（β-氨基乙基醚）-N，NN′,N′-四乙酸，β-巯基乙醇、谷胱甘肽、巯基乙酸和8-羟基喹啉抑制。
单位定义
一个胶原蛋白消化单元（CDU）从牛跟腱胶原蛋白中释放的肽链量和1.0 μ亮氨酸，5小时，pH7.4,37℃，在钙离子存在下的茚三酮颜色相同。一个 FALGPA水解单元每分钟，25℃，水解1.0 μmole 呋喃基丙烯酰-Leu-Gly-Pro-Ala。一个中性蛋白酶单元水解酪蛋白，产生颜色和1.0 μmole 酪氨酸每5小时，pH7.5,37℃下产生的相同。一个梭菌蛋白酶单元在DTT存在下，pH7.6,25℃，每分钟水解1.0 μmole BAEE。
分析说明
这些产品仅供测试和化验使用。它们不适用于人类或动物，也不能用于诊断、治疗或治愈任何类型的疾病。
其他说明
该产品是美国药典生物制品计划的一部分。
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
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