HPLC Analysis of MAb on Proteomix^® SCX-NP5 Comparing Different Buffer Systems and pH Gradients

技术

HPLC: suitable

试验参数

column: Proteomix SCX-NP5, 25 cm x 4.6 mm I.D., 5 μm particles (Z777161)
mobile phase: Mobile phase A: (A) 20 mM MES, pH 6.0; (B) A + 1 M NaCl, pH 6.0Mobile phase B: (A) 2.4 mM Tris, 1.5 mM imidazole, 11.6 mM piperazine, pH 6.0; (B) A, pH 10.5
gradient: Gradient A: 7% B held for 2 min; to 14% B in 20 min, Gradient B: 0 to 30% B in 2 min; to 55% B in 30 min
flow rate: 0.8 mL/min
detector: UV, 280 nm
injection: 5 μL
sample: 10 mg/mL

适用性

application for HPLC

应用

life science and biopharma
pharmaceutical (small molecule)