HPLC Analysis of HSA Fusion Protein on Proteomix^® SAX-NP5

技术

HPLC: suitable

试验参数

pressure: 2016 psi (139 bar)
column temp.: 30 °C
column: Proteomix SAX-NP5 25 cm x 4.6mm I.D., 5 μm particles (Z777229)
detector: UV, 280 nm
flow rate: 0.5 mL/min
mobile phase: Piperazine, Triethanolamine, bis-tris propane and N-methylpiperazine, 20 mM each,(A) pH 9.7; (B) pH 3.0 ( adjust by HCl )
sample: HSA fusion peptide 5 mg/mL, 10 μL (MW 75 kD, pI 5.0, HSA conjugated peptide in diabetes treatment)

适用性

application for HPLC

应用

life science and biopharma
pharmaceutical (small molecule)