HPLC Analysis of Recombinant Tumor Necrosis Factor Receptor-Fc Fusion Protein (rhTNFR-Fc) on Zenix^® SEC-300 versus SRT^® SEC-300, Effect of Particle Size on Resolution

Name: HPLC Analysis of Recombinant Tumor Necrosis Factor Receptor-Fc Fusion Protein (rhTNFR-Fc) on Zenix<SUP>®</SUP> SEC-300 versus SRT<SUP>®</SUP> SEC-300, Effect of Particle Size on Resolution
Brand: SUPELCO

application for HPLC

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技术

HPLC: suitable

试验参数

column: Zenix SEC-300, 30 cm x 7.8 mm I.D., 3 μm, 300 Å particles (Z777051)
column: SRT SEC-300, 30 cm x 7.8 mm I.D., 5 μm, 300 Å particles (Z777051) (Z777033)
mobile phase: 150 mM sodium phosphate, pH 7.0
flow rate: 1 mL/min
detector: UV, 214 nm
injection: 20 μL
sample: Recombinant tumor necrosis factor receptor-Fc fusion protein (rhTNFR-Fc), including oligomers (440 kD and 360 kD), monomer (150 kD), and impurity (120 kD)