HPLC Analysis of MAb 016 on Proteomix^® SCX NP5 versus Competitive SCX using a Salt Gradient

技术

HPLC: suitable

试验参数

column: Proteomix SCX-NP5, 25 cm x 4.6 mm I.D. (PEEK hardware), 5 μm particles (Z777164)
mobile phase: (A) 20 mM phosphate buffer, pH 7.5; (B) A + 1 M NaCl
gradient: 0 to 6% B in 30 min
flow rate: 0.8 mL/min
detector: UV, 280 nm
injection: 4 μL
sample: MAb 016 (11.8 mg/mL)

适用性

application for HPLC

应用

life science and biopharma
pharmaceutical (small molecule)