技术
HPLC: suitable
试验参数
column: Proteomix SCX-NP5, 25 cm x 4.6 mm I.D., 5 μm particles (Z777164)
mobile phase: [A] 2.4 mM Tris, 1.5 mM imidazole, 11.6 mM piperazine, pH 6.0; [B] A + 0.5 M NaCl, pH 10.5
gradient: top (0.8 mL/min): 5% B held for 5 min; to 29.5% B in 35 min; bottom (1.5 mL/min): 5% B held for 2.7 min; to 29.5% B in 18.6 min
flow rate: 0.8 mL/min or 1.5 mL/min
detector: UV 280 nm
injection: 20 μL
sample: MAb 321, 5 mg/mL
适用性
application for HPLC
应用
life science and biopharma
分析说明
Due to the low pressure of Proteomix SCX, faster analysis and high resolution can be achieved with a higher flow rate.
法律信息
Proteomix is a registered trademark of Sepax Technologies
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门