HPLC Analysis of Fab/Fc mAb Antibody Fragments on Proteomix^® WCX NP1.7

技术

HPLC: suitable

试验参数

column: Proteomix WCX-NP1.7, 3 cm x 4.6 mm I.D., 1.7 μm particles (Z777179)
mobile phase: (A) 20 mM sodium acetate, pH 5.15; (B) A + 1 M LiCl
gradient: 12% to 40% B in 6.7 min; to 100% B in 0.1 min; held for 2 min
flow rate: (1.5 mL/min)
pressure: 3335-3916 psi (230 - 270 bar)
detector: UV 280 nm
injection: 25 μL
sample: 1 mg/mL papain digested MAb 321

适用性

application for HPLC

应用

life science and biopharma
pharmaceutical (small molecule)