HPLC Analysis of Oligonucleotides on Proteomix^® SAX

技术

HPLC: suitable

试验参数

column: Proteomix SAX-NP5, 25 cm x 4.6 mm I.D., 5 μm particles (Z777229)
mobile phase: A: 20 mM Tris, pH 8.0 B: A + 0.5 M NaCl
gradient: 0 - 100% B in 30 minutes
flow rate: 0.5 mL/min
pressure: 1073 psi (74 bar)
column temp.: 25 °C
detector: UV 260 nm
injection: 5 μL
sample: (10 mM each oligonucleotide in water)

适用性

application for HPLC

应用

life science and biopharma
pharmaceutical (small molecule)