HPLC Analysis of Glycan and Isomers on Proteomix^® SAX-NP5, High Resolution Separation

技术

HPLC: suitable

试验参数

column: Proteomix SAX-NP5, 15 cm x 4.6 mm I.D., 5 μm particles (Z777228)
mobile phase: (A) 2.5% (v/v) acetic acid, 0.5% TEA in water; 0.5% acetic acid in acetonitrile
gradient: 0-100% B in 60 min
flow rate: 0.3 mL/min
detector: Fluorescence, Ex/Em=360/425 nm
sample: 2-AA (anthranilic acid) labeled N-linked oligosaccharide profiling of an IgG1 sample

适用性

application for HPLC

应用

life science and biopharma