HPLC Analysis of HSA Fusion Protein on Zenix^®-C SEC-300 vs. Zenix^® SEC-300

技术

HPLC: suitable

试验参数

column: Zenix SEC-300, 30 cm x 7.8 mm I.D., 3 μm particles (Z777033)
column: Zenix-C SEC-300, 30 cm x 7.8 mm I.D., 3 μm particles (Z777092)
mobile phase: 150 mM sodium phosphate buffer, pH 7.0
flow rate: 1 mL/min
column temp.: 25 °C
detector: UV, 280 nm
injection: 10 μL
sample: HSA fusion peptide 5 mg/mL (MW 75 kD, pl 5.0, HSA conjugated peptide in diabetes treatment)

适用性

application for HPLC

应用

life science and biopharma